IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/08
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Date: 08/08/13 People in lab: Blythe Ferriere
Title: Blue/white screening for norV TOPO-TA cloned colonies
Start Time: 4:10PM
Purpose: Plate screened for blue white colonies white colonies should have plasmid.
Protocol: Colonies from plate CT1 8/7/13 were inoculated into LB broth + ampicillin tubes
Notes: Ampicillin re-done in I1-I8
Stop Time: 5:41 PM
Next: Pick tubes with growth from white colonies for further analysis for check for norV gene
Date: 08/08/13 People in lab: Emily Puleo
Title: Gel electrophoresis of 8/7/13 PCR samples
Start Time: 4:37 PM
Purpose: To check for successful PCR reaction.
Protocol: LTM ed. 2 pg. 44