IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/08: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 68: Line 68:
<b>Protocol:</b> Invitrogen TOPO-TA cloning manual  
<b>Protocol:</b> Invitrogen TOPO-TA cloning manual  


<b>Products:</b>  
<b>Products:</b> {| border="1" cellpadding="5"
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| 8/8/13 hmp L1 || hmp in TOPO-TA cloning vector on ampicillin || 8/7/13 hmp A1 || 1
|}


<b>Notes:</b> Samples were 1 day old only one was completed because only one ampicillin plate was ready
<b>Notes:</b> Samples were 1 day old only one was completed because only one ampicillin plate was ready

Revision as of 14:07, 20 March 2014

Cyanobacteria Mediated Filtration of Coal Flue Gas <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Date: 08/08/13 People in lab: Blythe Ferriere

Title: Blue/white screening for norV TOPO-TA cloned colonies

Start Time: 4:10PM

Purpose: Plate screened for blue white colonies white colonies should have plasmid.

Protocol: Colonies from plate CT1 8/7/13 were inoculated into LB broth + ampicillin tubes

Products:

Sample Label Description Source Label Quantity
8/8/13 I 1 Isolation and inoculation of white colony 8/7/13 CT1 8
8/8/13 I 9 Isolation and inoculation of light blue colony 8/7/13 CT1 1
8/8/13 I 10 Isolation and inoculation of blue colony 8/7/13 CT1 3

Notes: Ampicillin re-done in I1-I8

Stop Time: 5:41 PM

Next: Pick tubes with growth from white colonies for further analysis for check for norV gene


Date: 08/08/13 People in lab: Emily Puleo

Title: Gel electrophoresis of 8/7/13 PCR samples

Start Time: 4:37 PM

Purpose: To check for successful PCR reaction.

Protocol: LTM ed. 2 pg. 44

Well 1 2 3 4 5 6 7 8
Sample Ladder 8/7/13 A norCB 8/7/13 A nosZ 1 8/7/13 A nosZ 2 8/7/13 A hmp 1 8/7/13 A hmp 2

Results: Product from hmp only. PICTURE

Notes: Used 70 uL sample+LoadingDye per well

Stop: 5:20PM

Date: 08/08/13 People in lab: Emily Puleo

Title: TOPO-TA cloning of 8/7/13 PCR samples

Start Time: 5:05 PM

Purpose: To put genes into vectors and transform into bacteria to amplify.

Protocol: Invitrogen TOPO-TA cloning manual

Products: {| border="1" cellpadding="5"

Sample Label Description Source Label Quantity
8/8/13 hmp L1 hmp in TOPO-TA cloning vector on ampicillin 8/7/13 hmp A1 1

Notes: Samples were 1 day old only one was completed because only one ampicillin plate was ready

Stop Time: 6:15 PM |}


Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue/2013/08/08&oldid=782476"