IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/20: Difference between revisions
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<b>Stop Time:</b> 10:15 AM | <b>Stop Time:</b> 10:15 AM | ||
== <b>Date: 8/20/13</b> <b>People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer</b> == | |||
<b><span style="font-size:16px;">Title:</span></b> PCR nosZ, norCB, and hmp to add iGEM prefix and suffix | |||
<b>Start Time:</b> 3:21PM | |||
<b>Purpose:</b> Make samples for GE and TOPO-TA cloning | |||
<b>Protocol:</b> LTM ed. 2 pg 48 PCR run conditions: Final primer concentration <0.1 uM, annealing temp: 65 C | |||
<b>Products:</b> | |||
{| border="1" cellpadding="5" | |||
|- | |||
! Sample Label !! Description !! Source Label !! Quantity | |||
|- | |||
| 8/20/13 norCB A || norCB w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 || P. aeru 7/30 || 1 | |||
|- | |||
| 8/20/13 nosZ A || nosZ w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 || P. aeru 7/30 || 1 | |||
|- | |||
| 8/20 hmp A || hmp w/ w/ iGEM prefix&suffix using 7.5uL template || E. coli 8/6 || 1 | |||
|- | |||
|} | |||
<b>Notes:</b> Products placed in freezer, no TOPO cloning | |||
<b>Stop Time:</b> 7:20 PM | |||
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Revision as of 17:08, 7 April 2014
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Date: 8/20/13 People in lab: Kelsey Crossen, Emily Puleo, Levi PalmerTitle: Making X-Gal Start Time: 9:30 AM Purpose: Making 1000x x-gal stock Protocol: 1. Add 1 mL of DMF to epi tube 2. Add 40 mg X-Gal to epi 3. Cover and shake to dissolve 4. Store in fridge(4 degrees) Products:
Stop Time: 10:15 AM Date: 8/20/13 People in lab: Kelsey Crossen, Emily Puleo, Levi PalmerTitle: PCR nosZ, norCB, and hmp to add iGEM prefix and suffix Start Time: 3:21PM Purpose: Make samples for GE and TOPO-TA cloning Protocol: LTM ed. 2 pg 48 PCR run conditions: Final primer concentration <0.1 uM, annealing temp: 65 C Products:
Notes: Products placed in freezer, no TOPO cloning Stop Time: 7:20 PM |