IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/20

From OpenWetWare

Jump to: navigation, search
(Date: MM/DD/YY People in lab: Yourname)
Current revision (20:08, 7 April 2014) (view source)
(Date: 8/20/13 People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer)
 
Line 27: Line 27:
<b>Stop Time:</b> 10:15 AM
<b>Stop Time:</b> 10:15 AM
 +
== <b>Date: 8/20/13</b> <b>People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer</b>  ==
 +
<b><span style="font-size:16px;">Title:</span></b> PCR nosZ, norCB, and hmp to add iGEM prefix and suffix
 +
 +
<b>Start Time:</b> 3:21PM
 +
 +
<b>Purpose:</b> Make samples for GE and TOPO-TA cloning
 +
 +
<b>Protocol:</b> LTM ed. 2 pg 48 PCR run conditions: Final primer concentration <0.1 uM, annealing temp: 65 C
 +
 +
<b>Products:</b>
 +
{| border="1" cellpadding="5"
 +
|-
 +
! Sample Label !! Description !! Source Label !! Quantity
 +
|-
 +
| 8/20/13 norCB A || norCB w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 || P. aeru 7/30 || 1
 +
|-
 +
| 8/20/13 nosZ A || nosZ w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 || P. aeru 7/30 || 1
 +
|-
 +
| 8/20 hmp A || hmp w/ w/ iGEM prefix&suffix using 7.5uL template || E. coli 8/6 || 1
 +
|-
 +
|}
 +
 +
<b>Notes:</b> Products placed in freezer, no TOPO cloning
 +
 +
<b>Stop Time:</b> 7:20 PM
<!-- ## Do not edit below this line unless you know what you are doing. ## -->
<!-- ## Do not edit below this line unless you know what you are doing. ## -->

Current revision

Cyanobacteria Mediated Filtration of Coal Flue Gas Main project page
Previous entry      Next entry

Date: 8/20/13 People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer

Title: Making X-Gal

Start Time: 9:30 AM

Purpose: Making 1000x x-gal stock

Protocol: 1. Add 1 mL of DMF to epi tube 2. Add 40 mg X-Gal to epi 3. Cover and shake to dissolve 4. Store in fridge(4 degrees)

Products:

Sample Label Description Source Label Quantity
X-Gal 8/20 40 mg/mL xgal (1000x), 1 mL X-Gal, solid 1

Stop Time: 10:15 AM

Date: 8/20/13 People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer

Title: PCR nosZ, norCB, and hmp to add iGEM prefix and suffix

Start Time: 3:21PM

Purpose: Make samples for GE and TOPO-TA cloning

Protocol: LTM ed. 2 pg 48 PCR run conditions: Final primer concentration <0.1 uM, annealing temp: 65 C

Products:

Sample Label Description Source Label Quantity
8/20/13 norCB A norCB w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 P. aeru 7/30 1
8/20/13 nosZ A nosZ w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 P. aeru 7/30 1
8/20 hmp A hmp w/ w/ iGEM prefix&suffix using 7.5uL template E. coli 8/6 1

Notes: Products placed in freezer, no TOPO cloning

Stop Time: 7:20 PM


Personal tools