IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/26: Difference between revisions

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(Autocreate 2013/08/26 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
== <b>Date: 08/26/13</b> <b>People in lab: Emily Puleo, Hannah Frye</b>  ==


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b><span style="font-size:16px;">Title:</span></b> PCR norCB and nosZ


<b>Start Time:</b>
<b>Start Time:</b> 1:00 PM


<b>Purpose:</b>  
<b>Purpose:</b> Adjust PCR program to attempt to get product


<b>Protocol:</b> <b>Exceptions:</b>  
<b>Protocol:</b> LTM ed. 2 <b>Exceptions:</b> 1. Annealing Temp: 67C 2. Elongation Time: 7 min


<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border="1" cellpadding="5"
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description  
|-
|-
| Entry || Entry || Entry || #
| 8/24 norCB A || norCB with iGEM prefix&suffix ||
|-
|-
| Entry || Entry || Entry || #
| 8/24 nosZ A || nosZ with iGEM prefix&suffix ||  
|}
|}


{| border="1" cellpadding="5"
<b>Notes:</b> See 7/31 notes for calculations
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
|-
| Sample ||  ||  ||  ||  ||  ||  ||  ||
|}
 
<b>Results:</b>  
 
<b>Notes:</b>


<b>Stop Time:</b>  
<b>Stop Time:</b> 6:15 PM


<b>Next:</b>
<b>Next:</b> Gel electrophoresis




Revision as of 20:40, 16 May 2014

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Date: 08/26/13 People in lab: Emily Puleo, Hannah Frye

Title: PCR norCB and nosZ

Start Time: 1:00 PM

Purpose: Adjust PCR program to attempt to get product

Protocol: LTM ed. 2 Exceptions: 1. Annealing Temp: 67C 2. Elongation Time: 7 min

Products:

Sample Label Description
8/24 norCB A norCB with iGEM prefix&suffix
8/24 nosZ A nosZ with iGEM prefix&suffix

Notes: See 7/31 notes for calculations

Stop Time: 6:15 PM

Next: Gel electrophoresis



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