IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/09/28: Difference between revisions

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(Autocreate 2013/09/28 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
== <b>Date: 09/28/13</b> <b>People in lab: Levi Palmer</b>  ==


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b><span style="font-size:16px;">Title:</span></b> Digest backbone, ligate into circular plasmid


<b>Start Time:</b>
<b>Start Time:</b> 1:16 PM


<b>Purpose:</b>  
<b>Purpose:</b> To circularize the linear backbones


<b>Protocol:</b> <b>Exceptions:</b>
<b>Protocol:</b> LTM ed. 2


<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border="1" cellpadding="5"
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description  
|-
|-
| Entry || Entry || Entry || #
| D LP 9/28 || Digested ampicillin backbone with E and P
|-
|-
| Entry || Entry || Entry || #
| L1 LP 9/28 || Circular ampicillin backbone 
|}
|}


{| border="1" cellpadding="5"
<b>Notes:</b> IMPORTANT: Don't do this, it won't work. Must digest with S and X, not E and P! -LP 11/21/13
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
|-
| Sample ||  ||  ||  ||  ||  ||  ||  ||
|}
 
<b>Results:</b>  
 
<b>Notes:</b>


<b>Stop Time:</b>  
<b>Stop Time:</b> 3:30 PM


<b>Next:</b>




Revision as of 11:47, 17 May 2014

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Date: 09/28/13 People in lab: Levi Palmer

Title: Digest backbone, ligate into circular plasmid

Start Time: 1:16 PM

Purpose: To circularize the linear backbones

Protocol: LTM ed. 2

Products:

Sample Label Description
D LP 9/28 Digested ampicillin backbone with E and P
L1 LP 9/28 Circular ampicillin backbone

Notes: IMPORTANT: Don't do this, it won't work. Must digest with S and X, not E and P! -LP 11/21/13

Stop Time: 3:30 PM




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