IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/09/30: Difference between revisions
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Levi Palmer (talk | contribs) (Autocreate 2013/09/30 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue) |
Levi Palmer (talk | contribs) |
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== <b>Date: | == <b>Date: 09/30/13</b> <b>People in lab: Levi Palmer</b> == | ||
<b><span style="font-size:16px;">Title:</span></b> | <b><span style="font-size:16px;">Title:</span></b> Transform backbone into competent cells | ||
<b>Start Time:</b> | <b>Start Time:</b> 4:12 PM | ||
<b>Purpose:</b> | <b>Purpose:</b> To test for competence of cells and amplify amp backbone | ||
<b>Protocol:</b> | <b>Protocol:</b> LTM ed. 2 | ||
<b>Products:</b> | <b>Products:</b> | ||
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! Sample Label !! Description !! Source Label !! Quantity | ! Sample Label !! Description !! Source Label !! Quantity | ||
|- | |- | ||
| | | 9/30 Amp Backbone CT || E. coli transformed with Amp backbone || L LP 9/28 || 2 | ||
|- | |- | ||
| | | 9/30 pUC19 BB CT || E. coli transformed with pUC19 control plasmid || pUC 19 DNA || 2 | ||
|} | |} | ||
<b>Results:</b> 10/01: Transformations did not work. | |||
<b>Results:</b> | |||
<b>Notes:</b> | <b>Notes:</b> Performed with both amp and pUC 19 plasmids | ||
<b>Stop Time:</b> | <b>Stop Time:</b> 7:00 PM | ||
<b>Next:</b> | <b>Next:</b> Check for success, if succesful, inoculate amp cells into LB broth. | ||
Revision as of 11:58, 17 May 2014
 Cyanobacteria Mediated Filtration of Coal Flue Gas
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Date: 09/30/13 People in lab: Levi PalmerTitle: Transform backbone into competent cells Start Time: 4:12 PM Purpose: To test for competence of cells and amplify amp backbone Protocol: LTM ed. 2 Products:
Results: 10/01: Transformations did not work. Notes: Performed with both amp and pUC 19 plasmids Stop Time: 7:00 PM Next: Check for success, if succesful, inoculate amp cells into LB broth.
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