IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/11/02: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Making Competent Cells==
==Making Competent Cells==
Persons participating: Levi Palmer, Alie Abele, Emily Puleo, Kelsey Crossen
Persons participating: Levi Palmer, Emily Puleo, Kelsey Crossen
* 9am - Will make chemically competent DH5a cells.  
* 9am - Will make chemically competent DH5a cells.  


== <b>Date: 11/2/13</b> <b>People in lab: Levi Palmer</b>  ==
<b><span style="font-size:16px;">Title:</span></b> Test 11/2 competent cells for competence
<b>Start Time:</b> 5:48 PM
<b>Purpose:</b> To test for competence of cells by transformation
<b>Protocol:</b> LTM ed. 2
<b>Products:</b>
{| border="1" cellpadding="5"
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| 11/2/13 G1 control CT || 11/2 competent cells with pUC19, not grown on antibiotic, positive control || 11/2 G1 pUC19 CT || 4
|-
| 11/2/13 G1 pUC19 CT || 11/2 competent cells with pUC19, grown on Ampicillin || 11/2 G5 pUC19 CT || 4
|}
<b>Results:</b> Unsure results, lawn growth, retest with negative control
<b>Stop Time:</b> 9:00 PM




Latest revision as of 23:32, 26 September 2017

iGEM Project name 1 Main project page
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Making Competent Cells

Persons participating: Levi Palmer, Emily Puleo, Kelsey Crossen

  • 9am - Will make chemically competent DH5a cells.

Date: 11/2/13 People in lab: Levi Palmer

Title: Test 11/2 competent cells for competence

Start Time: 5:48 PM

Purpose: To test for competence of cells by transformation

Protocol: LTM ed. 2

Products:

Sample Label Description Source Label Quantity
11/2/13 G1 control CT 11/2 competent cells with pUC19, not grown on antibiotic, positive control 11/2 G1 pUC19 CT 4
11/2/13 G1 pUC19 CT 11/2 competent cells with pUC19, grown on Ampicillin 11/2 G5 pUC19 CT 4

Results: Unsure results, lawn growth, retest with negative control

Stop Time: 9:00 PM



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