IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/11/08

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(Entry title)
Current revision (14:17, 19 November 2013) (view source)
(Date:11/08/13 People in lab: Levi Palmer)
 
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== <b>Date:</b>11/08/13 <b>People in lab:</b> Levi Palmer ==
== <b>Date:</b>11/08/13 <b>People in lab:</b> Levi Palmer ==
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Title: Chemical Transformation test for competency
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<b>Title</b>: Chemical Transformation test for competency
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Start Time: 6:00 PM
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<u>Start Time</u>: 6:00 PM
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Purpose: To test 11/1/13 Chemically Competent cells for competency.
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<u>Purpose</u>: To test 11/1/13 Chemically Competent cells for competency.
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Protocol: iGEM LTM ed. 2 Pages: 41-43 <u>Exceptions:</u> 1. Only used 1 μL of pUC19 plasmid. 2. Negative and positive controls were made.
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<u>Protocol</u>: iGEM LTM ed. 2 Pages: 41-43 <u>Exceptions:</u> 1. Only used 1 μL of pUC19 plasmid. 2. Negative and positive controls were made.
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Products:  
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<u>Products</u>:  
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<u>Sample Label:</u> CT pUC19 11/8/13 1  <u>Description:</u> Cells transformed with pUC19  <u>Source Label:</u> pUC19, Chem. Comp. Cells 11/1/13
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<u>Sample Label</u>: CT pUC19 11/8/13 1  <u>Description</u>: Cells transformed with pUC19  <u>Source Label</u>: pUC19, Chem. Comp. Cells 11/1/13
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<u>Sample Label:</u> CT pUC19 11/8/13 2  <u>Description:</u> Cells transformed with pUC19  <u>Source Label:</u> pUC19, Chem. Comp. Cells 11/1/13
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<u>Sample Label</u>: CT pUC19 11/8/13 2  <u>Description</u>: Cells transformed with pUC19  <u>Source Label</u>: pUC19, Chem. Comp. Cells 11/1/13
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<u>Sample Label:</u> Negative control    <u>Description:</u> Untransformed cells          <u>Source Label:</u> Chem. Comp. cells 11/1/13
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<u>Sample Label</u>: Negative control    <u>Description</u>: Untransformed cells          <u>Source Label</u>: Chem. Comp. cells 11/1/13
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<u>Sample Label:</u> Positive control    <u>Description:</u> Cells transformed with pUC19, no Amp. <u>Source Label:</u> pUC19, Chem. Comp. Cells 11/1/13
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<u>Sample Label</u>: Positive control    <u>Description</u>: Cells transformed with pUC19, no Amp. <u>Source Label</u>: pUC19, Chem. Comp. Cells 11/1/13
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Results: Hopefully the cells were competent!
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<u>Results</u>: Plates did not have enough ampicillin, negative control grew.
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Notes: Est. efficiency of 94%
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<u>Notes</u>:  
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Stop Time: 9:00 PM
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<u>Stop Time</u>: 9:00 PM
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Next: USE THESE CELLS, USE THEM ALL!
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<u>Next</u>: Make more amp plates.

Current revision

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Date:11/08/13 People in lab: Levi Palmer

Title: Chemical Transformation test for competency

Start Time: 6:00 PM

Purpose: To test 11/1/13 Chemically Competent cells for competency.

Protocol: iGEM LTM ed. 2 Pages: 41-43 Exceptions: 1. Only used 1 μL of pUC19 plasmid. 2. Negative and positive controls were made.

Products:

Sample Label: CT pUC19 11/8/13 1 Description: Cells transformed with pUC19 Source Label: pUC19, Chem. Comp. Cells 11/1/13

Sample Label: CT pUC19 11/8/13 2 Description: Cells transformed with pUC19 Source Label: pUC19, Chem. Comp. Cells 11/1/13

Sample Label: Negative control Description: Untransformed cells Source Label: Chem. Comp. cells 11/1/13

Sample Label: Positive control Description: Cells transformed with pUC19, no Amp. Source Label: pUC19, Chem. Comp. Cells 11/1/13

Results: Plates did not have enough ampicillin, negative control grew.

Notes:

Stop Time: 9:00 PM

Next: Make more amp plates.




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