IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/11/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> iGEM Project name 1</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== <b>Date:</b>11/08/13 <b>People in lab:</b> Levi Palmer == | == <b>Date:</b>11/08/13 <b>People in lab:</b> Levi Palmer == | ||
Title: Chemical Transformation test for competency | <b>Title</b>: Chemical Transformation test for competency | ||
Start Time: 6:00 PM | <u>Start Time</u>: 6:00 PM | ||
Purpose: To test 11/1/13 Chemically Competent cells for competency. | <u>Purpose</u>: To test 11/1/13 Chemically Competent cells for competency. | ||
Protocol: iGEM LTM ed. 2 Pages: 41-43 <u>Exceptions:</u> 1. Only used 1 μL of pUC19 plasmid. 2. Negative and positive controls were made. | <u>Protocol</u>: iGEM LTM ed. 2 Pages: 41-43 <u>Exceptions:</u> 1. Only used 1 μL of pUC19 plasmid. 2. Negative and positive controls were made. | ||
Products: | <u>Products</u>: | ||
<u>Sample Label | <u>Sample Label</u>: CT pUC19 11/8/13 1 <u>Description</u>: Cells transformed with pUC19 <u>Source Label</u>: pUC19, Chem. Comp. Cells 11/1/13 | ||
<u>Sample Label | <u>Sample Label</u>: CT pUC19 11/8/13 2 <u>Description</u>: Cells transformed with pUC19 <u>Source Label</u>: pUC19, Chem. Comp. Cells 11/1/13 | ||
<u>Sample Label | <u>Sample Label</u>: Negative control <u>Description</u>: Untransformed cells <u>Source Label</u>: Chem. Comp. cells 11/1/13 | ||
<u>Sample Label | <u>Sample Label</u>: Positive control <u>Description</u>: Cells transformed with pUC19, no Amp. <u>Source Label</u>: pUC19, Chem. Comp. Cells 11/1/13 | ||
Results: | <u>Results</u>: Plates did not have enough ampicillin, negative control grew. | ||
Notes: | <u>Notes</u>: | ||
Stop Time: 9:00 PM | <u>Stop Time</u>: 9:00 PM | ||
Next: | <u>Next</u>: Make more amp plates. | ||
Latest revision as of 23:34, 26 September 2017
 iGEM Project name 1
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Date:11/08/13 People in lab: Levi PalmerTitle: Chemical Transformation test for competency Start Time: 6:00 PM Purpose: To test 11/1/13 Chemically Competent cells for competency. Protocol: iGEM LTM ed. 2 Pages: 41-43 Exceptions: 1. Only used 1 μL of pUC19 plasmid. 2. Negative and positive controls were made. Products: Sample Label: CT pUC19 11/8/13 1 Description: Cells transformed with pUC19 Source Label: pUC19, Chem. Comp. Cells 11/1/13 Sample Label: CT pUC19 11/8/13 2 Description: Cells transformed with pUC19 Source Label: pUC19, Chem. Comp. Cells 11/1/13 Sample Label: Negative control Description: Untransformed cells Source Label: Chem. Comp. cells 11/1/13 Sample Label: Positive control Description: Cells transformed with pUC19, no Amp. Source Label: pUC19, Chem. Comp. Cells 11/1/13 Results: Plates did not have enough ampicillin, negative control grew. Notes: Stop Time: 9:00 PM Next: Make more amp plates.
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