IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/11/08: Difference between revisions
Levi Palmer (talk | contribs) (Autocreate 2013/11/08 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue) |
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== | == <b>Date:</b>11/08/13 <b>People in lab:</b> Levi Palmer == | ||
Title: Chemical Transformation test for competency | |||
Start Time: 6:00 PM | |||
Purpose: To test 11/1/13 Chemically Competent cells for competency. | |||
Protocol: iGEM LTM ed. 2 Pages: 41-43 <u>Exceptions:</u> 1. Only used 1 μL of pUC19 plasmid. 2. Negative and positive controls were made. | |||
Products: | |||
<u>Sample Label:</u> CT pUC19 11/8/13 1 <u>Description:</u> Cells transformed with pUC19 <u>Source Label:</u> pUC19, Chem. Comp. Cells 11/1/13 | |||
<u>Sample Label:</u> CT pUC19 11/8/13 2 <u>Description:</u> Cells transformed with pUC19 <u>Source Label:</u> pUC19, Chem. Comp. Cells 11/1/13 | |||
<u>Sample Label:</u> Negative control <u>Description:</u> Untransformed cells <u>Source Label:</u> Chem. Comp. cells 11/1/13 | |||
<u>Sample Label:</u> Positive control <u>Description:</u> Cells transformed with pUC19, no Amp. <u>Source Label:</u> pUC19, Chem. Comp. Cells 11/1/13 | |||
Results: Hopefully the cells were competent! | |||
Notes: Est. efficiency of 94% | |||
Stop Time: 9:00 PM | |||
Next: USE THESE CELLS, USE THEM ALL! | |||
Revision as of 17:31, 8 November 2013
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Date:11/08/13 People in lab: Levi PalmerTitle: Chemical Transformation test for competency Start Time: 6:00 PM Purpose: To test 11/1/13 Chemically Competent cells for competency. Protocol: iGEM LTM ed. 2 Pages: 41-43 Exceptions: 1. Only used 1 μL of pUC19 plasmid. 2. Negative and positive controls were made. Products: Sample Label: CT pUC19 11/8/13 1 Description: Cells transformed with pUC19 Source Label: pUC19, Chem. Comp. Cells 11/1/13 Sample Label: CT pUC19 11/8/13 2 Description: Cells transformed with pUC19 Source Label: pUC19, Chem. Comp. Cells 11/1/13 Sample Label: Negative control Description: Untransformed cells Source Label: Chem. Comp. cells 11/1/13 Sample Label: Positive control Description: Cells transformed with pUC19, no Amp. Source Label: pUC19, Chem. Comp. Cells 11/1/13 Results: Hopefully the cells were competent! Notes: Est. efficiency of 94% Stop Time: 9:00 PM Next: USE THESE CELLS, USE THEM ALL!
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