IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/11/08

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Date:11/08/13 People in lab: Levi Palmer

Title: Chemical Transformation test for competency

Start Time: 6:00 PM

Purpose: To test 11/1/13 Chemically Competent cells for competency.

Protocol: iGEM LTM ed. 2 Pages: 41-43 Exceptions: 1. Only used 1 μL of pUC19 plasmid. 2. Negative and positive controls were made.

Products:

Sample Label: CT pUC19 11/8/13 1 Description: Cells transformed with pUC19 Source Label: pUC19, Chem. Comp. Cells 11/1/13

Sample Label: CT pUC19 11/8/13 2 Description: Cells transformed with pUC19 Source Label: pUC19, Chem. Comp. Cells 11/1/13

Sample Label: Negative control Description: Untransformed cells Source Label: Chem. Comp. cells 11/1/13

Sample Label: Positive control Description: Cells transformed with pUC19, no Amp. Source Label: pUC19, Chem. Comp. Cells 11/1/13

Results: Plates did not have enough ampicillin, negative control grew.

Notes:

Stop Time: 9:00 PM

Next: Make more amp plates.




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