IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/11/18: Difference between revisions

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(Autocreate 2013/11/18 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
== <b>Date: 11/18/13</b> <b>People in lab: Blythe Ferriere</b>  ==


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b><span style="font-size:16px;">Title:</span></b> PCR norV for PstI digestion


<b>Start Time:</b>
<b>Start Time:</b> 9:00 AM


<b>Purpose:</b>  
<b>Purpose:</b> To amplify the norV gene before PCR mutagenesis to remove the PstI site


<b>Protocol:</b> <b>Exceptions:</b>
<b>Protocol:</b> LTM ed. 2


<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border="1" cellpadding="5"
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Quantity
|-
|-
| Entry || Entry || Entry || #
| 11/18/13 norV A || norV with iGEM prefix/suffix || 2
|-
| Entry || Entry || Entry || #
|}
|}


{| border="1" cellpadding="5"
<b>Notes:</b> Used norCB program on PCR machine
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
|-
| Sample ||  ||  ||  ||  ||  ||  ||  ||
|}
 
<b>Results:</b>
 
<b>Notes:</b>  


<b>Stop Time:</b>  
<b>Stop Time:</b> 3:00 PM


<b>Next:</b>
<b>Next:</b> Digest with PstI




Revision as of 16:25, 17 May 2014

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Date: 11/18/13 People in lab: Blythe Ferriere

Title: PCR norV for PstI digestion

Start Time: 9:00 AM

Purpose: To amplify the norV gene before PCR mutagenesis to remove the PstI site

Protocol: LTM ed. 2

Products:

Sample Label Description Quantity
11/18/13 norV A norV with iGEM prefix/suffix 2

Notes: Used norCB program on PCR machine

Stop Time: 3:00 PM

Next: Digest with PstI



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