IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/02/05: Difference between revisions
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Levi Palmer (talk | contribs) (Autocreate 2014/02/05 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue) |
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== <b>Date: | == <b>Date: 02/5/14</b> <b>People in lab: Kelsey Crossen</b> == | ||
<b><span style="font-size:16px;">Title:</span></b> | <b><span style="font-size:16px;">Title:</span></b> PCR of nosZ, adding prefix/suffix | ||
<b>Start Time:</b> | <b>Start Time:</b> 12:05 PM | ||
<b>Purpose:</b> | <b>Purpose:</b> To amplify the nosZ gene and add the iGEM prefix/suffix | ||
<b>Protocol:</b> <b>Exceptions:</b> | <b>Protocol:</b> LTM ed. 2 and Dr. Shannon's lab PCR protocol <b>Exceptions:</b> 1. Annealing temp: 50C, 2. Final primer conc. <0.1uM | ||
<b>Products:</b> | <b>Products:</b> | ||
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! Sample Label !! Description !! Source Label !! Quantity | ! Sample Label !! Description !! Source Label !! Quantity | ||
|- | |- | ||
| | | nosZ 1 || LTM protocol amplified nosZ || P aeru 7//31 || 1 | ||
|- | |- | ||
| | | nosZ 2 || Dr. Shannon lab protocol amplified nosZ || P aeru 7/31 || 1 | ||
|} | |} | ||
<b>Notes:</b> Dr Shannon's Protocol: 7.5 uL template, 3 uL DreamTaq buffer, 3 uL dNTPs, 2.5 uL F primer, 2.5 uL R primer, 10.5 uL milliQ, 1 uL DreamTaq. Hot start (95C) then add Taq. | |||
<b> | |||
<b>Stop Time:</b> | <b>Stop Time:</b> | ||
<b>Next:</b> | <b>Next:</b> Gel electrophoresis of PCR product | ||
Revision as of 17:52, 17 May 2014
 Cyanobacteria Mediated Filtration of Coal Flue Gas
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Date: 02/5/14 People in lab: Kelsey CrossenTitle: PCR of nosZ, adding prefix/suffix Start Time: 12:05 PM Purpose: To amplify the nosZ gene and add the iGEM prefix/suffix Protocol: LTM ed. 2 and Dr. Shannon's lab PCR protocol Exceptions: 1. Annealing temp: 50C, 2. Final primer conc. <0.1uM Products:
Notes: Dr Shannon's Protocol: 7.5 uL template, 3 uL DreamTaq buffer, 3 uL dNTPs, 2.5 uL F primer, 2.5 uL R primer, 10.5 uL milliQ, 1 uL DreamTaq. Hot start (95C) then add Taq. Stop Time: Next: Gel electrophoresis of PCR product
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