IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/02/27: Difference between revisions
Levi Palmer (talk | contribs) (Autocreate 2014/02/27 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue) |
Levi Palmer (talk | contribs) |
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== <b>Date: | == <b>Date: 02/27/14</b> <b>People in lab: Levi Palmer</b> == | ||
<b><span style="font-size:16px;">Title:</span></b> | <b><span style="font-size:16px;">Title:</span></b> Blue white colony screening of hmp | ||
<b>Start Time:</b> | <b>Start Time:</b> 11:00 AM | ||
<b>Purpose:</b> | <b>Purpose:</b> To confirm colonies have hmp gene | ||
<b>Protocol:</b> | <b>Protocol:</b> 1. Add 15 uL of 1000x X-gal to LB Amp plates 2. Plate 0.2 uL overnight culture 3. Add 15 uL SOC to plate to help spread cells 4. Spread with beads 5. Incubate overnight 37C | ||
<b>Products:</b> | <b>Products:</b> | ||
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! Sample Label !! Description !! Source Label !! Quantity | ! Sample Label !! Description !! Source Label !! Quantity | ||
|- | |- | ||
| | | 2/27 Poss hmp Xgal LP || Transformed cells on amp+xgal || Poss hmp LP 2/26 || 1 | ||
|} | |} | ||
<b>Results:</b> 2/28/14: Lawn growth, unusable for B/W colony screening | |||
<b>Notes:</b> | |||
<b>Stop Time:</b> 12:00 PM | |||
<b>Next:</b> Inoculate any white colonies in LB Amp broth | |||
== <b>Date: 02/27/14</b> <b>People in lab: Levi Palmer</b> == | |||
<b><span style="font-size:16px;">Title:</span></b> norCB primer dilution | |||
<b>Start Time:</b> 12:00 PM | |||
<b>Purpose:</b> To prepare new primers for PCR, bring down to ~0.7 uM | |||
<b>Protocol:</b> 1. To make 1 ug/uL stock, add 430 uL milliQ to 0.43 mg F primer, and 400uL to .4 mg R primer, 2. To make (1X) working solution, add 1uL stock to 99uL milliQ for final concentration of 10 ng/uL or ~0.7 uM | |||
<b>Products:</b> | |||
{| border="1" cellpadding="5" | {| border="1" cellpadding="5" | ||
|- | |- | ||
! | ! Sample Label !! Description !! Source Label !! Quantity | ||
|- | |- | ||
| | | 2/27 F norCB primer || 0.7 uM F primer norCB || IDT norCB F 2/24 || 1 | ||
|} | |- | ||
| 2/27 R norCB primer || 0.7 uM R primer norCB || IDT norCB R 2/24 || 1 | |||
|} | |||
<b> | <b>Notes:</b> Stored in -20C freezer in norCB box | ||
<b>Stop Time:</b> 1:00 PM | |||
<b>Next:</b> Inoculate any white colonies in LB Amp broth | |||
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Revision as of 18:31, 17 May 2014
 Cyanobacteria Mediated Filtration of Coal Flue Gas
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Date: 02/27/14 People in lab: Levi PalmerTitle: Blue white colony screening of hmp Start Time: 11:00 AM Purpose: To confirm colonies have hmp gene Protocol: 1. Add 15 uL of 1000x X-gal to LB Amp plates 2. Plate 0.2 uL overnight culture 3. Add 15 uL SOC to plate to help spread cells 4. Spread with beads 5. Incubate overnight 37C Products:
Results: 2/28/14: Lawn growth, unusable for B/W colony screening Notes: Stop Time: 12:00 PM Next: Inoculate any white colonies in LB Amp broth Date: 02/27/14 People in lab: Levi PalmerTitle: norCB primer dilution Start Time: 12:00 PM Purpose: To prepare new primers for PCR, bring down to ~0.7 uM Protocol: 1. To make 1 ug/uL stock, add 430 uL milliQ to 0.43 mg F primer, and 400uL to .4 mg R primer, 2. To make (1X) working solution, add 1uL stock to 99uL milliQ for final concentration of 10 ng/uL or ~0.7 uM Products:
Notes: Stored in -20C freezer in norCB box Stop Time: 1:00 PM Next: Inoculate any white colonies in LB Amp broth | |||||||||||||||||||||
