IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/03/11: Difference between revisions

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(Autocreate 2014/03/11 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
== <b>Date: 03/11/14</b> <b>People in lab: Levi Palmer</b>  ==


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b><span style="font-size:16px;">Title:</span></b> Gel electrophoresis of 3/10 Digest


<b>Start Time:</b>
<b>Start Time:</b> 11:00 AM


<b>Purpose:</b>  
<b>Purpose:</b> To check for presence of hmp gene in digest and TOPO vector


<b>Protocol:</b> <b>Exceptions:</b>  
<b>Protocol:</b> LTM ed. 2
 
<b>Results:</b> No hmp present, no bands present, no picture taken
 
<b>Next:</b> Retry hmp PCR and TOPO cloning
 
== <b>Date: 03/11/14</b> <b>People in lab: Levi Palmer</b>  ==
 
<b><span style="font-size:16px;">Title:</span></b> Making chemically competent cells
 
<b>Purpose:</b> To make chemical competent cells to use in lab.
 
<b>Protocol:</b> OpenWetWare: Making Chemically Competent Cells (Inoue) <b>Exceptions:</b> 1. O.D. <0.1 of SOB grown culture 2. Adjusted to volume of 250mL 3. 1/2 volume of final resuspension


<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border="1" cellpadding="5"
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label
|-
| Entry || Entry || Entry || #
|-
|-
| Entry || Entry || Entry || #
| Chemical Competent 3/11/14 || Chemically competent DH5alpha, in -80C || DH5alpha stock
|}
|}


{| border="1" cellpadding="5"
<b>Notes:</b> Test for competence was successful.
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
|-
| Sample ||  ||  ||  ||  ||  ||  ||  ||
|}
 
<b>Results:</b>
 
<b>Notes:</b>  
 
<b>Stop Time:</b>
 
<b>Next:</b>




Revision as of 21:37, 17 May 2014

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Date: 03/11/14 People in lab: Levi Palmer

Title: Gel electrophoresis of 3/10 Digest

Start Time: 11:00 AM

Purpose: To check for presence of hmp gene in digest and TOPO vector

Protocol: LTM ed. 2

Results: No hmp present, no bands present, no picture taken

Next: Retry hmp PCR and TOPO cloning

Date: 03/11/14 People in lab: Levi Palmer

Title: Making chemically competent cells

Purpose: To make chemical competent cells to use in lab.

Protocol: OpenWetWare: Making Chemically Competent Cells (Inoue) Exceptions: 1. O.D. <0.1 of SOB grown culture 2. Adjusted to volume of 250mL 3. 1/2 volume of final resuspension

Products:

Sample Label Description Source Label
Chemical Competent 3/11/14 Chemically competent DH5alpha, in -80C DH5alpha stock

Notes: Test for competence was successful.



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