IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/04/14: Difference between revisions

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(Autocreate 2014/04/14 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
== <b>Date: 04/14/14</b> <b>People in lab: Kelsey Crossen</b>  ==


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b><span style="font-size:16px;">Title:</span></b> PCR norCB


<b>Start Time:</b>
<b>Start Time:</b> 2:30PM


<b>Purpose:</b>  
<b>Purpose:</b> To amplify norCB gene from P. aeruginosa


<b>Protocol:</b> <b>Exceptions:</b>  
<b>Protocol:</b> LTM ed. 2  <b>Exceptions:</b> 1. Annealing temp: 55C


<b>Products:</b>  
<b>Products:</b>  
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! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity
|-
|-
| Entry || Entry || Entry || #
| norCB 4/14 || Amplified norCB with prefix and suffix || P aeru 2 3/3 || 1
|-
| Entry || Entry || Entry || #
|}
|}
{| border="1" cellpadding="5"
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
|-
| Sample ||  ||  ||  ||  ||  ||  ||  ||
|}


<b>Results:</b>  
<b>Results:</b>  
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<b>Notes:</b>  
<b>Notes:</b>  


<b>Stop Time:</b>  
<b>Stop Time:</b> 5:00 PM


<b>Next:</b>
<b>Next:</b> Run gel to check for PCR product




Revision as of 22:16, 17 May 2014

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Date: 04/14/14 People in lab: Kelsey Crossen

Title: PCR norCB

Start Time: 2:30PM

Purpose: To amplify norCB gene from P. aeruginosa

Protocol: LTM ed. 2 Exceptions: 1. Annealing temp: 55C

Products:

Sample Label Description Source Label Quantity
norCB 4/14 Amplified norCB with prefix and suffix P aeru 2 3/3 1

Results:

Notes:

Stop Time: 5:00 PM

Next: Run gel to check for PCR product



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