IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/04/14: Difference between revisions
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== <b>Date: 04/14/14</b> <b>People in lab: Kelsey Crossen</b> == | |||
<b><span style="font-size:16px;">Title:</span></b> Gel electrophoresis of norCB PCR | |||
<b>Start Time:</b> 6:50 PM | |||
<b>Purpose:</b> To check for successful PCR | |||
<b>Protocol:</b> LTM ed. 2 | |||
{| border="1" cellpadding="5" | |||
|- | |||
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8 | |||
|- | |||
| Sample || Ladder || norCB 4/14 || || || || || || | |||
|} | |||
<b>Results:</b> No PCR product visible | |||
<b>Stop Time:</b> 8:00 PM | |||
<b>Next:</b> Try low temp PCR (annealing Temp 40C) | |||
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Revision as of 22:19, 17 May 2014
 Cyanobacteria Mediated Filtration of Coal Flue Gas
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Date: 04/14/14 People in lab: Kelsey CrossenTitle: PCR norCB Start Time: 2:30PM Purpose: To amplify norCB gene from P. aeruginosa Protocol: LTM ed. 2 Exceptions: 1. Annealing temp: 55C Products:
Results: Notes: Stop Time: 5:00 PM Next: Run gel to check for PCR product
Date: 04/14/14 People in lab: Kelsey CrossenTitle: Gel electrophoresis of norCB PCR Start Time: 6:50 PM Purpose: To check for successful PCR Protocol: LTM ed. 2
Results: No PCR product visible Stop Time: 8:00 PM Next: Try low temp PCR (annealing Temp 40C) | |||||||||||||||||||||||||||
