IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/04/20: Difference between revisions

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== <b>Date: 8/20/13</b> <b>People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer</b>  ==


<b><span style="font-size:16px;">Title:</span></b> Making X-Gal


<b>Start Time:</b> 9:30 AM


<b>Purpose:</b> Making 1000x x-gal stock
<b>Protocol:</b> 1. Add 1 mL of DMF to epi tube 2. Add 40 mg X-Gal to epi 3. Cover and shake to dissolve 4. Store in fridge(4 degrees)
<b>Products:</b>
{| border="1" cellpadding="5"
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| X-Gal 8/20 || 40 mg/mL xgal (1000x), 1 mL || X-Gal, solid || 1
|-
|}
<b>Stop Time:</b> 10:15 AM
== <b>Date: 8/20/13</b> <b>People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer</b>  ==
<b><span style="font-size:16px;">Title:</span></b> PCR nosZ, norCB, and hmp to add iGEM prefix and suffix
<b>Start Time:</b> 3:21PM
<b>Purpose:</b> Make samples for GE and TOPO-TA cloning
<b>Protocol:</b> LTM ed. 2 pg 48 PCR run conditions: Final primer concentration <0.1 uM, annealing temp: 65 C
<b>Products:</b>
{| border="1" cellpadding="5"
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| 8/20/13 norCB A || norCB w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 || P. aeru 7/30 || 1
|-
| 8/20/13 nosZ A || nosZ w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 || P. aeru 7/30 || 1
|-
| 8/20 hmp A || hmp w/ w/ iGEM prefix&suffix using 7.5uL template || E. coli 8/6 || 1
|-
|}
<b>Notes:</b> Products placed in freezer, no TOPO cloning
<b>Stop Time:</b> 7:20 PM




Revision as of 17:09, 7 April 2014

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