IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/04/20: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 8: Line 8:




== <b>Date: 8/20/13</b> <b>People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer</b>  ==


<b><span style="font-size:16px;">Title:</span></b> PCR nosZ, norCB, and hmp to add iGEM prefix and suffix
<b>Start Time:</b> 3:21PM
<b>Purpose:</b> Make samples for GE and TOPO-TA cloning
<b>Protocol:</b> LTM ed. 2 pg 48 PCR run conditions: Final primer concentration <0.1 uM, annealing temp: 65 C
<b>Products:</b>
{| border="1" cellpadding="5"
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| 8/20/13 norCB A || norCB w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 || P. aeru 7/30 || 1
|-
| 8/20/13 nosZ A || nosZ w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 || P. aeru 7/30 || 1
|-
| 8/20 hmp A || hmp w/ w/ iGEM prefix&suffix using 7.5uL template || E. coli 8/6 || 1
|-
|}
<b>Notes:</b> Products placed in freezer, no TOPO cloning
<b>Stop Time:</b> 7:20 PM




Revision as of 17:09, 7 April 2014

Cyanobacteria Mediated Filtration of Coal Flue Gas <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>





Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue/2014/04/20&oldid=786474"