IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/04/20

From OpenWetWare

Jump to: navigation, search
(Date: 8/20/13 People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer)
Current revision (20:09, 7 April 2014) (view source)
(Date: 8/20/13 People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer)
 
Line 8: Line 8:
-
== <b>Date: 8/20/13</b> <b>People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer</b>  ==
 
-
<b><span style="font-size:16px;">Title:</span></b> PCR nosZ, norCB, and hmp to add iGEM prefix and suffix
 
-
 
-
<b>Start Time:</b> 3:21PM
 
-
 
-
<b>Purpose:</b> Make samples for GE and TOPO-TA cloning
 
-
 
-
<b>Protocol:</b> LTM ed. 2 pg 48 PCR run conditions: Final primer concentration <0.1 uM, annealing temp: 65 C
 
-
 
-
<b>Products:</b>
 
-
{| border="1" cellpadding="5"
 
-
|-
 
-
! Sample Label !! Description !! Source Label !! Quantity
 
-
|-
 
-
| 8/20/13 norCB A || norCB w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 || P. aeru 7/30 || 1
 
-
|-
 
-
| 8/20/13 nosZ A || nosZ w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 || P. aeru 7/30 || 1
 
-
|-
 
-
| 8/20 hmp A || hmp w/ w/ iGEM prefix&suffix using 7.5uL template || E. coli 8/6 || 1
 
-
|-
 
-
|}
 
-
 
-
<b>Notes:</b> Products placed in freezer, no TOPO cloning
 
-
 
-
<b>Stop Time:</b> 7:20 PM
 

Current revision

Cyanobacteria Mediated Filtration of Coal Flue Gas Main project page
Previous entry      Next entry





Personal tools