IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/04/20

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Date: 8/20/13 People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer

Title: Making X-Gal

Start Time: 9:30 AM

Purpose: Making 1000x x-gal stock

Protocol: 1. Add 1 mL of DMF to epi tube 2. Add 40 mg X-Gal to epi 3. Cover and shake to dissolve 4. Store in fridge(4 degrees)

Products:

Sample Label Description Source Label Quantity
X-Gal 8/20 40 mg/mL xgal (1000x), 1 mL X-Gal, solid 1

Stop Time: 10:15 AM

Date: 8/20/13 People in lab: Kelsey Crossen, Emily Puleo, Levi Palmer

Title: PCR nosZ, norCB, and hmp to add iGEM prefix and suffix

Start Time: 3:21PM

Purpose: Make samples for GE and TOPO-TA cloning

Protocol: LTM ed. 2 pg 48 PCR run conditions: Final primer concentration <0.1 uM, annealing temp: 65 C

Products:

Sample Label Description Source Label Quantity
8/20/13 norCB A norCB w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 P. aeru 7/30 1
8/20/13 nosZ A nosZ w/ iGEM prefix&suffix using 7.5uL template in 0.5uL h20 P. aeru 7/30 1
8/20 hmp A hmp w/ w/ iGEM prefix&suffix using 7.5uL template E. coli 8/6 1

Notes: Products placed in freezer, no TOPO cloning

Stop Time: 7:20 PM



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