IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/07/24

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Date: 7/24/14 People in lab: Emily Puleo

Title: PCR hmp Start Time: 12:03 pm Purpose: Add prefix and suffix Protocol: LTM Ed 2 PCR Exceptions: 2.5 uL primers, 7.5 uL E coli Template Products:

Sample Label Description Source Label Quantity
7/24 A hmp with prefix and suffix added --- 1





Results: Notes: Stop Time: 3:10 pm Next: TOPO

Date: 7/24/14 People in lab: Emily Puleo, Caleb Treccazi

Title: TOPO cloning of hmp and transformations of hmp and norV Start Time: 3:10 pm Purpose: To get lots of copies of hmp and do blue white screening and to test ligated norV product Protocol: LTM Ed 2 Transformations, Invitrogen TOPO cloning kit manual Exceptions: Products:

Sample Label Description Source Label Quantity
7/24 CT hmp 1 20 uL hmp and 60 uL xgal 7/24 A 1
7/24 CT hmp 2 200 uL hmp and 60 uL xgal 7/24 A 1
7/24 CT norV 1 20 uL L1 6/6 1
7/24 CT norV 2 200 uL L1 6/6 1


Results: (7/25) No growth except on hmp 1 where there were lots of white colonies and one blue colony Notes: Stop Time: 5:45 pm Next: blue white screening of hmp; innoculation of norV from any growth



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