IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/08/03: Difference between revisions

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(Autocreate 2014/08/03 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b> ==
==<b>Date: 8/3/14</b> <b>People in lab: Emily Puleo, Joshua Heath</b>==
 
<b><span style="font-size:16px;">Title:</span></b> Chemical Transformation
<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b>Start Time:</b> 5 pm
 
<b>Purpose:</b> Prepare backbone (chlor resistance with promoter and RBS)
<b>Start Time:</b>
<b>Protocol:</b> LTM Ed. 2 Chemical Transformations
 
<b>Exceptions:</b>  
<b>Purpose:</b>  
 
<b>Protocol:</b> <b>Exceptions:</b>  
 
<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border= " 1 " cellpadding = " 5 "  
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity  
|-
|-
| Entry || Entry || Entry || #
| 2014 1-30 || BBa_K608002 || Plate 1 2014 kit well 30 || 1
|-
|-
| Entry || Entry || Entry || #
| 2014 3-3F || BBa_B0015 || Plate 3 2014 kit well 3F || 1
|}
|-
 
| 8/3 CT1 || 20 uL || 2014 1-30 || 1
{| border="1" cellpadding="5"
|-
| 8/3 CT2 || 200 uL || 2014 1-30 || 1
|-
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
| 8/3 CT3 || 20 uL || 2014 3-3F || 1
|-
|-
| Sample ||  || || ||  ||  ||  ||  ||  
| 8/3 CT4 || 200 uL || 2014 3-3F || 1
|}  
|}
 
<b>Results:</b> no growth
<b>Results:</b>  
 
<b>Notes:</b>  
<b>Notes:</b>  
 
<b>Stop Time:</b> 8 pm
<b>Stop Time:</b>  
<b>Next:</b> Try again with different comp cells
 
<b>Next:</b>




Revision as of 23:00, 13 October 2014

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Date: 8/3/14 People in lab: Emily Puleo, Joshua Heath

Title: Chemical Transformation Start Time: 5 pm Purpose: Prepare backbone (chlor resistance with promoter and RBS) Protocol: LTM Ed. 2 Chemical Transformations Exceptions: Products:

Sample Label Description Source Label Quantity
2014 1-30 BBa_K608002 Plate 1 2014 kit well 30 1
2014 3-3F BBa_B0015 Plate 3 2014 kit well 3F 1
8/3 CT1 20 uL 2014 1-30 1
8/3 CT2 200 uL 2014 1-30 1
8/3 CT3 20 uL 2014 3-3F 1
8/3 CT4 200 uL 2014 3-3F 1

Results: no growth Notes: Stop Time: 8 pm Next: Try again with different comp cells



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