IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/08/11
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Date: 8/11/14 People in lab: Emily Puleo, Joshua HeathTitle: Gel Extraction of hmp Start Time: 1 pm Purpose: Isolate hmp from vector Protocol: LTM Ed. 2 Gel Extraction Exceptions: Products:
Results: Notes: Stop Time: 1:45 pm Next: Ligate with pro+rbs part Date: 8/11/14 People in lab: Emily Puleo, Joshua HeathTitle: Digest pro+RBS and TT Start Time: 1:45 pm Purpose: Prep for ligation Protocol: LTM Ed. 2 Digest Exceptions: For pro+RBS = 15.5 uL DNA, 5 uL milliQ, 1 uL speI and pstI; for TT = 1 uL xbal and pstI Products:
Results: Notes: Stop Time: 3:45 pm Next: Nanodrop Date: 8/11/14 People in lab: Emily Puleo, Joshua HeathTitle: Nanodrop Start Time: 3:55 pm Purpose: Measure the purity and concentration of the samples Protocol: LTM Ed. 2 Nanodrop Exceptions: Products:
Results: Notes: Stop Time: 4:05 pm Next: Ligation
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