IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/08/19: Difference between revisions

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(Autocreate 2014/08/19 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b> ==
==<b>Date: 8/19/14</b> <b>People in lab: Emily Puleo, Kelsey Crossen</b>==
 
<b><span style="font-size:16px;">Title:</span></b> Chemical Transformation
<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b>Start Time:</b> 3:45 pm
 
<b>Purpose:</b> To transform ligated product into comp cells
<b>Start Time:</b>
<b>Protocol:</b> LTM Ed. 2 Chemical Transformation
 
<b>Exceptions:</b>  
<b>Purpose:</b>  
 
<b>Protocol:</b> <b>Exceptions:</b>  
 
<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border= " 1 " cellpadding = " 5 "  
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity  
|-
|-
| Entry || Entry || Entry || #
| CT1 8/19 || 20 uL || L1 8/18 || 1
|-
|-
| Entry || Entry || Entry || #
| CT2 8/19 || 200 uL || L1 8/18 || 1
|}
 
{| border="1" cellpadding="5"
|-
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
| CT3 8/19 || 20 uL || L1 8/18 || 1
|-
|-
| Sample || || || ||  ||  ||  ||  ||
| CT4 8/19 || 200 uL || L1 8/8 || 1
|}


<b>Results:</b>


|}
<b>Results: (8/20) no growth present, only misleading air bubbles that looked like colonies; retry with differences in vector/insert ratio or new buffer</b>
<b>Notes:</b>  
<b>Notes:</b>  
 
<b>Stop Time:</b> 6:14 pm
<b>Stop Time:</b>  
<b>Next:</b> Start liquid cultures from colonies that grow
 
<b>Next:</b>
 
 


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Revision as of 14:31, 14 October 2014

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Date: 8/19/14 People in lab: Emily Puleo, Kelsey Crossen

Title: Chemical Transformation Start Time: 3:45 pm Purpose: To transform ligated product into comp cells Protocol: LTM Ed. 2 Chemical Transformation Exceptions: Products:

Sample Label Description Source Label Quantity
CT1 8/19 20 uL L1 8/18 1
CT2 8/19 200 uL L1 8/18 1
CT3 8/19 20 uL L1 8/18 1
CT4 8/19 200 uL L1 8/8 1


Results: (8/20) no growth present, only misleading air bubbles that looked like colonies; retry with differences in vector/insert ratio or new buffer Notes: Stop Time: 6:14 pm Next: Start liquid cultures from colonies that grow


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