IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/08/27: Difference between revisions

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(Autocreate 2014/08/27 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b> ==
==<b>Date: 8/27/14</b> <b>People in lab: Kelsey Crossen</b>==
 
<b><span style="font-size:16px;">Title:</span></b> Transformation of hmp and pro+RBS backbone and RFP in chlor resistant backbone to test comp cells
<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b>Start Time:</b> 2 pm
 
<b>Purpose:</b> To test if comp cells are working by running a control with the target reaction for the project
<b>Start Time:</b>
<b>Protocol:</b> LTM Ed. 2 Chemical Transformation
 
<b>Exceptions:</b> 1.5 uL RFP and 1.5 uL Ligation product used and run in the same transformation
<b>Purpose:</b>  
 
<b>Protocol:</b> <b>Exceptions:</b>  
 
<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border= " 1 " cellpadding = " 5 "  
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity  
|-
|-
| Entry || Entry || Entry || #
| CT1 8/27 || 20 uL || L1 8/18 and DH5 alpha 2013 DJW || 1
|-
|-
| Entry || Entry || Entry || #
| CT2 8/27 || 200 uL || L1 8/18 and DH5 alpha 2013 DJW || 1
|}
 
{| border="1" cellpadding="5"
|-
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
| CT3 8/27 || 20 uL || RFP in chlor bb stock 12/18/13 || 1
|-
|-
| Sample || || || ||  ||  ||  ||  ||
| CT4 8/27 || 200 uL || RFP in chlor bb stock 12/18/13 || 1
|}


<b>Results:</b>
<b>Notes:</b>
<b>Stop Time:</b>
<b>Next:</b>


|}
<b>Results:</b> (8/29) No growth - comp cells are deemed bad
<b>Notes:</b> DH5 alpha cells used from bottom shelf of -80 C freezer
<b>Stop Time:</b> 5 pm
<b>Next:</b> Comp cells need to be remade




Revision as of 14:33, 14 October 2014

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Date: 8/27/14 People in lab: Kelsey Crossen

Title: Transformation of hmp and pro+RBS backbone and RFP in chlor resistant backbone to test comp cells Start Time: 2 pm Purpose: To test if comp cells are working by running a control with the target reaction for the project Protocol: LTM Ed. 2 Chemical Transformation Exceptions: 1.5 uL RFP and 1.5 uL Ligation product used and run in the same transformation Products:

Sample Label Description Source Label Quantity
CT1 8/27 20 uL L1 8/18 and DH5 alpha 2013 DJW 1
CT2 8/27 200 uL L1 8/18 and DH5 alpha 2013 DJW 1
CT3 8/27 20 uL RFP in chlor bb stock 12/18/13 1
CT4 8/27 200 uL RFP in chlor bb stock 12/18/13 1


Results: (8/29) No growth - comp cells are deemed bad Notes: DH5 alpha cells used from bottom shelf of -80 C freezer Stop Time: 5 pm Next: Comp cells need to be remade



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