IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/09/25: Difference between revisions

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(Autocreate 2014/09/25 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b> ==
==<b>Date: 9/25/14</b> <b>People in lab: Kelsey Crossen</b>==
 
<b><span style="font-size:16px;">Title:</span></b> Digestions of hmp and vectors
<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b>Start Time:</b> 2 pm
 
<b>Purpose:</b> Digest hmp from TOPO vector, chlor bb with pro+RBS and iGEM plasmid (psB1C3)
<b>Start Time:</b>
<b>Protocol:</b> LTM Ed. 2 Digestion
 
<b>Exceptions:</b> psB1C3 digestion done at half value due to low concentration
<b>Purpose:</b>  
 
<b>Protocol:</b> <b>Exceptions:</b>  
 
<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border= " 1 " cellpadding = " 5 "  
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity  
|-
|-
| Entry || Entry || Entry || #
| D1 9/25 || hmp digested with E and P || MP4 7/30 || 1
|-
|-
| Entry || Entry || Entry || #
| D2 9/25 || hmp digested with E and P || MP4 7/30 || 1
|}
 
{| border="1" cellpadding="5"
|-
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
| D3 9/25 || pro+RBS bacbone digested with S and P || MP2 8/9 || 1
|-
|-
| Sample || || || ||  ||  ||  ||  ||
| D4 9/25 || shipping backbone digested with E and P || 2013 psB1C3 || 1
|}


|}
<b>Results:</b>  
<b>Results:</b>  
<b>Notes:</b> D1 needs to be ligated into D4 and D2 needs to be ligated into D3
<b>Stop Time:</b> 4:25 pm
<b>Next:</b>


<b>Notes:</b>
<b>Stop Time:</b>
<b>Next:</b>




Revision as of 19:22, 14 October 2014

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Date: 9/25/14 People in lab: Kelsey Crossen

Title: Digestions of hmp and vectors Start Time: 2 pm Purpose: Digest hmp from TOPO vector, chlor bb with pro+RBS and iGEM plasmid (psB1C3) Protocol: LTM Ed. 2 Digestion Exceptions: psB1C3 digestion done at half value due to low concentration Products:

Sample Label Description Source Label Quantity
D1 9/25 hmp digested with E and P MP4 7/30 1
D2 9/25 hmp digested with E and P MP4 7/30 1
D3 9/25 pro+RBS bacbone digested with S and P MP2 8/9 1
D4 9/25 shipping backbone digested with E and P 2013 psB1C3 1


Results: Notes: D1 needs to be ligated into D4 and D2 needs to be ligated into D3 Stop Time: 4:25 pm Next:




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