IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/09/29: Difference between revisions

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(Autocreate 2014/09/29 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b> ==
==<b>Date: 9/29/14</b> <b>People in lab: Kira Buckowing</b>==
<b><span style="font-size:16px;">Title:</span></b> Minipreps
<b>Start Time:</b> 12:15 pm
<b>Purpose:</b> To purify the wanted plasimd from liquid cultures
<b>Protocol:</b> LTM Ed. 2 Miniprep
<b>Exceptions:</b>
<b>Products:</b>
{| border= " 1 " cellpadding = " 5 "
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| MP1 9/29 || Purrified hmp plasmid || BC1 9/28 || 1
|-
| MP2 9/29  || Purified hmp plasmid || BC6 9/28 || 1


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.


<b>Start Time:</b>


<b>Purpose:</b>


<b>Protocol:</b> <b>Exceptions:</b>


|}
<b>Results:</b>
<b>Notes:</b>
<b>Stop Time:</b> 2:20 pm
<b>Next:</b> Digest of plasmid and gel to see if a vector and insert part are clearly visible
==<b>Date: 9/29/14</b> <b>People in lab: Kelsey Crossen</b>==
<b><span style="font-size:16px;">Title:</span></b> Nanodrop of miniprep product
<b>Start Time:</b> 3:15 pm
<b>Purpose:</b> Find out concentration of miniprepped hmp plasmids
<b>Protocol:</b> LTM Ed. 2 Nanodrop
<b>Exceptions:</b>
<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border= " 1 " cellpadding = " 5 "  
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity  
|-
|-
| Entry || Entry || Entry || #
| --- || ng/uL = 42.4; 260/280 = 2.13; 260/230 = 2.11 || MP1 9/29 || 1
|-
|-
| Entry || Entry || Entry || #
| --- || ng/uL = 26.2; 260/280 = 2.02; 260/230 = 1.61 || MP2 9/29 || 1
 
 
 
 
 
 
|}
|}
<b>Results:</b>
<b>Notes:</b>
<b>Stop Time:</b> 3:30 pm
<b>Next:</b> Digest to confirm presence of hmp


{| border="1" cellpadding="5"
==<b>Date: 9/29/14</b> <b>People in lab: Kelsey Crossen</b>==
<b><span style="font-size:16px;">Title:</span></b> Digest miniprep plasmids to confirm identity
<b>Start Time:</b> 3:30 pm
<b>Purpose:</b> To confirm the presence of hmp in the ligated plasmid
<b>Protocol:</b> LTM Ed. 2 Digest and Gels
<b>Exceptions:</b>
<b>Products:</b>
{| border= " 1 " cellpadding = " 5 "  
|-
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
|-
|-
| Sample || || || || || ||  ||  ||  
| - || Ladder || - || D1 || - || D2 ||  ||  ||
|}  
|}
 
<b>Products:</b>
<b>Results:</b>  
<b>Results:</b> Two bands visible for D1 ~1100 bp and ~2200 bp, correspondign to hmp and psB1C3 bacbone
 
<b>Notes:</b>  
<b>Notes:</b>  
 
<b>Stop Time:</b> 5:50 pm
<b>Stop Time:</b>  
<b>Next:</b> Re-transform miniprep product into comp cells to make glycerol stocks
 
<b>Next:</b>
 




Revision as of 19:40, 14 October 2014

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Date: 9/29/14 People in lab: Kira Buckowing

Title: Minipreps Start Time: 12:15 pm Purpose: To purify the wanted plasimd from liquid cultures Protocol: LTM Ed. 2 Miniprep Exceptions: Products:

Sample Label Description Source Label Quantity
MP1 9/29 Purrified hmp plasmid BC1 9/28 1
MP2 9/29 Purified hmp plasmid BC6 9/28 1




Results: Notes: Stop Time: 2:20 pm Next: Digest of plasmid and gel to see if a vector and insert part are clearly visible

Date: 9/29/14 People in lab: Kelsey Crossen

Title: Nanodrop of miniprep product Start Time: 3:15 pm Purpose: Find out concentration of miniprepped hmp plasmids Protocol: LTM Ed. 2 Nanodrop Exceptions: Products:

Sample Label Description Source Label Quantity
--- ng/uL = 42.4; 260/280 = 2.13; 260/230 = 2.11 MP1 9/29 1
--- ng/uL = 26.2; 260/280 = 2.02; 260/230 = 1.61 MP2 9/29 1




Results: Notes: Stop Time: 3:30 pm Next: Digest to confirm presence of hmp

Date: 9/29/14 People in lab: Kelsey Crossen

Title: Digest miniprep plasmids to confirm identity Start Time: 3:30 pm Purpose: To confirm the presence of hmp in the ligated plasmid Protocol: LTM Ed. 2 Digest and Gels Exceptions: Products:

Well 1 2 3 4 5 6 7 8
- Ladder - D1 - D2

Products: Results: Two bands visible for D1 ~1100 bp and ~2200 bp, correspondign to hmp and psB1C3 bacbone Notes: Stop Time: 5:50 pm Next: Re-transform miniprep product into comp cells to make glycerol stocks



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