IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Output/2009/06/15/Min Lin
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9:00
MiniPrep 1-12M and 1-12O.
Digest with EcoRI and XbaI
| EcoRI | 1μL |
| XbaI | 1μL |
| 10xM buffer | 5μL |
| Plasmid | 43μL |
Transformation (pSalSer plasmid from Voigt Lab into Top10)
22:00
Digest 1-12M and 1-12O with EcoRI and SpeI overnight.
| XbaI | 1μL |
| PstI | 1μL |
| 10xM buffer | 5μL |
| Plasmid | 43μL |
Digest T7 promoter with
| SpeI | 1μL |
| PstI | 1μL |
| 10xH buffer | 5μL |
| Plasmid | 43μL |
