IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/16/Shan Shen

11:30
Electrophoresis to test the PCR products.
The order and the amount of the samples: marker 5μL, bi—stable PCR products(from 50 to 61 centigrade)
Results:

All the products are correct according to the gel picture.

12:30
Recycle the correct bands.

13:10
PCR high-copy bi-stable system plasmids.
The system is the same with the high-copy plasmids.

16:30
Electrophoresis to test the PCR products.
The order and the amount of the samples: marker 5μL, bi—stable PCR products, control
Results:
PCR is unsuccessful.