IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/07/28
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Cell cultureWe cultured cells from the pKP33-aompF-GFPuv + pBad-aompF-7.9 double transformation in 10 ml of : plain LB, LB+1%glucose and LB+1%arabinose. Colony PCRresults of the colony PCR from yesterday the clones 8, 9 and 10 are inoculated in liquid culture (LB+kan). They will be miniprepped and rechecked by PCR. |