IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/07/31: Difference between revisions
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(Autocreate 2012/07/31 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/Delay_group/day_by_day/) |
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== | ==Results of the double transformation in top 10 cells== | ||
Positive control : OK | |||
pBad-aOmpF-7.9 : OK | |||
pKP33-aOmpF-GFPuv : OK | |||
pKP33-GFPuv : OK, no fluo | |||
pBad-aOmpF-7.9 + pKP33-aOmpF-GFPuv : OK, but no fluo | |||
pBad-aOmpF-7.9 + pKP33-GFPuv : no colony | |||
==Digestion of the clone 8-9-10 (ligation pLac-RBS-GFP-TT + araC-pBad-RBS-lacI) == | |||
We digest 1µg of them in 10µL with EcoRI and PstI (1µL each) | |||
Result : only clone 9 shows a band at 2500bp | |||
Revision as of 08:27, 7 August 2012
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Results of the double transformation in top 10 cellsPositive control : OK pBad-aOmpF-7.9 : OK pKP33-aOmpF-GFPuv : OK pKP33-GFPuv : OK, no fluo pBad-aOmpF-7.9 + pKP33-aOmpF-GFPuv : OK, but no fluo pBad-aOmpF-7.9 + pKP33-GFPuv : no colony Digestion of the clone 8-9-10 (ligation pLac-RBS-GFP-TT + araC-pBad-RBS-lacI)We digest 1µg of them in 10µL with EcoRI and PstI (1µL each) Result : only clone 9 shows a band at 2500bp
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