IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/07/26
From OpenWetWare
(→Recovery) |
(→Digestion) |
||
| Line 57: | Line 57: | ||
The insert is purified on a 0.8% agarose gel. We obtain a concentration of 24.1 ng/µl | The insert is purified on a 0.8% agarose gel. We obtain a concentration of 24.1 ng/µl | ||
| - | [[Image:KR003886.png| | + | [[Image:KR003886.png|left|400px]] |
| + | [[Image:KR003887.png|right|400px]] | ||
The vector is purified wth the PCR kit. We obtain a concentration of 86.4 ng/µl | The vector is purified wth the PCR kit. We obtain a concentration of 86.4 ng/µl | ||
Revision as of 12:59, 26 July 2012
 Project name
|  Main project page Previous entry Next entry
|
Recovery1- pLac-RBS-GFP-TT clone 1 (From Antoine) +IPTG+Amp 2- pLac-RBS-GFP-TT clone 1 (From Antoine) +Amp 3- pLac-RBS-GFP-TT clone 1 (From Antoine) +IPTG+Kan 4- pLac-RBS-GFP-TT clone 1 (From Antoine) +Kan 5- pLac-RBS-GFP-TT clone 1 (From Denis) + IPTG + Amp 6- pLac-RBS-GFP-TT clone 1 (From Denis) + Amp 7- araC-pBad-RBS-LacI clone 2 (from the 2009 Paris iGEM team) + Amp 8- araC-pBad-RBS-LacI clone 2 (from the 2009 Paris iGEM team) + Kan 9- pLac-RBS-GFP-TT clone 2 (From Antoine) +IPTG+Amp 10- pLac-RBS-GFP-TT clone 2 (From Antoine) +Amp 11- pLac-RBS-GFP-TT clone 4 (From Antoine) +IPTG+Amp 12- pLac-RBS-GFP-TT clone 4 (From Antoine) +Amp We'll need to do a glycerol stock for the strains that worked. Digestioninsert = pLac-GFP-TT vector = araC-pBad-RBS-LacI The insert is digested in E&S, the vector is digested in E&X. Digestion of the insert :
Digestion of the vector :
The insert is purified on a 0.8% agarose gel. We obtain a concentration of 24.1 ng/µl   The vector is purified wth the PCR kit. We obtain a concentration of 86.4 ng/µl | |
