IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/07/31

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(Autocreate 2012/07/31 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/Delay_group/day_by_day/)
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==Entry title==
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==Results of the double transformation in top 10 cells==
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Positive control : OK
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pBad-aOmpF-7.9 : OK
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pKP33-aOmpF-GFPuv : OK
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pKP33-GFPuv : OK, no fluo
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pBad-aOmpF-7.9 + pKP33-aOmpF-GFPuv : OK, but no fluo
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pBad-aOmpF-7.9 + pKP33-GFPuv : no colony
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==Digestion of the clone 8-9-10 (ligation pLac-RBS-GFP-TT + araC-pBad-RBS-lacI) ==
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We digest 1µg of them in 10µL with EcoRI and PstI (1µL each)
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Result : only clone 9 shows a band at 2500bp

Revision as of 11:27, 7 August 2012

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Results of the double transformation in top 10 cells

Positive control : OK

pBad-aOmpF-7.9 : OK

pKP33-aOmpF-GFPuv : OK

pKP33-GFPuv : OK, no fluo

pBad-aOmpF-7.9 + pKP33-aOmpF-GFPuv : OK, but no fluo

pBad-aOmpF-7.9 + pKP33-GFPuv : no colony

Digestion of the clone 8-9-10 (ligation pLac-RBS-GFP-TT + araC-pBad-RBS-lacI)

We digest 1µg of them in 10µL with EcoRI and PstI (1µL each)

Result : only clone 9 shows a band at 2500bp


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