IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/07/31
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Current revision (11:29, 7 August 2012) (view source) (→Digestion of the clone 8-9-10 (ligation pLac-RBS-GFP-TT + araC-pBad-RBS-lacI)) |
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Result : only clone 9 shows a band at 2500bp | Result : only clone 9 shows a band at 2500bp | ||
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Results of the double transformation in top 10 cellsPositive control : OK pBad-aOmpF-7.9 : OK pKP33-aOmpF-GFPuv : OK pKP33-GFPuv : OK, no fluo pBad-aOmpF-7.9 + pKP33-aOmpF-GFPuv : OK, but no fluo pBad-aOmpF-7.9 + pKP33-GFPuv : no colony Digestion of the clone 8-9-10 (ligation pLac-RBS-GFP-TT + araC-pBad-RBS-lacI)We digest 1µg of them in 10µL with EcoRI and PstI (1µL each) Result : only clone 9 shows a band at 2500bp
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