IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/07/31: Difference between revisions
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Result : only clone 9 shows a band at 2500bp | Result : only clone 9 shows a band at 2500bp | ||
[[Image:KR003918.png]] | |||
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Revision as of 08:29, 7 August 2012
 Project name
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Results of the double transformation in top 10 cellsPositive control : OK pBad-aOmpF-7.9 : OK pKP33-aOmpF-GFPuv : OK pKP33-GFPuv : OK, no fluo pBad-aOmpF-7.9 + pKP33-aOmpF-GFPuv : OK, but no fluo pBad-aOmpF-7.9 + pKP33-GFPuv : no colony Digestion of the clone 8-9-10 (ligation pLac-RBS-GFP-TT + araC-pBad-RBS-lacI)We digest 1µg of them in 10µL with EcoRI and PstI (1µL each) Result : only clone 9 shows a band at 2500bp
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