IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/10/09: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2012/10/09 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/Delay_group/day_by_day/)
 
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Entry title==
* Insert content here...
Back from Amsterdam!
 
We received the primers for the biobricking of the sRNA Delay system.
*PCR
We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.
 
1° sRNA  amplified from strain #27. Expected product : 1746bp
2° ColE2  amplified from strain #5.   Expected product : 1801bp
3° aOmpF amplified from strain #25    Expected product : 159bp
4° YiaGp  amplified from MG1655    Expected product : 136bp
 
Tm : 52°C, elongation time = 1min, phusion.
 
We run the PCR product on 2 gels : -1% agarose for 1°&2°
-2% agarose for 3°&4°





Revision as of 05:04, 9 October 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>

Entry title

Back from Amsterdam!

We received the primers for the biobricking of the sRNA Delay system.

  • PCR

We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.

1° sRNA amplified from strain #27. Expected product : 1746bp 2° ColE2 amplified from strain #5. Expected product : 1801bp 3° aOmpF amplified from strain #25 Expected product : 159bp 4° YiaGp amplified from MG1655 Expected product : 136bp

Tm : 52°C, elongation time = 1min, phusion.

We run the PCR product on 2 gels : -1% agarose for 1°&2° -2% agarose for 3°&4°