IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/10/09
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(Autocreate 2012/10/09 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/Delay_group/day_by_day/) |
Current revision (08:09, 9 October 2012) (view source) (→Entry title) |
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| - | == | + | Back from Amsterdam! |
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| + | We received the primers for the biobricking of the sRNA Delay system. | ||
| + | ==PCR== | ||
| + | We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp. | ||
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| + | 1° sRNA amplified from strain #27. Expected product : 1746bp | ||
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| + | 2° ColE2 amplified from strain #5. Expected product : 1801bp | ||
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| + | 3° aOmpF amplified from strain #25 Expected product : 159bp | ||
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| + | 4° YiaGp amplified from MG1655 Expected product : 136bp | ||
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| + | Tm : 52°C, elongation time = 1min, phusion. | ||
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| + | We run the PCR product on 2 gels : -1% agarose for 1°&2° | ||
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| + | -2% agarose for 3°&4° | ||
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Back from Amsterdam! We received the primers for the biobricking of the sRNA Delay system. PCRWe amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.
2° ColE2 amplified from strain #5. Expected product : 1801bp 3° aOmpF amplified from strain #25 Expected product : 159bp 4° YiaGp amplified from MG1655 Expected product : 136bp Tm : 52°C, elongation time = 1min, phusion. We run the PCR product on 2 gels : -1% agarose for 1°&2° -2% agarose for 3°&4°
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