IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/10/09

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(Autocreate 2012/10/09 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/Delay_group/day_by_day/)
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==Entry title==
==Entry title==
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Back from Amsterdam!
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We received the primers for the biobricking of the sRNA Delay system.
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*PCR
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We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.
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1° sRNA  amplified from strain #27. Expected product : 1746bp
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2° ColE2  amplified from strain #5.   Expected product : 1801bp
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3° aOmpF amplified from strain #25    Expected product : 159bp
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4° YiaGp  amplified from MG1655    Expected product : 136bp
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Tm : 52°C, elongation time = 1min, phusion.
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We run the PCR product on 2 gels : -1% agarose for 1°&2°
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-2% agarose for 3°&4°

Revision as of 08:04, 9 October 2012

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Entry title

Back from Amsterdam!

We received the primers for the biobricking of the sRNA Delay system.

  • PCR

We amplify the following products : sRNA (the sRNA generating part of the pBAD sRNA 7.9 plasmid), ColE2 (without RBS), aOmpF (stop before ATG) and YiaGp.

1° sRNA amplified from strain #27. Expected product : 1746bp 2° ColE2 amplified from strain #5. Expected product : 1801bp 3° aOmpF amplified from strain #25 Expected product : 159bp 4° YiaGp amplified from MG1655 Expected product : 136bp

Tm : 52°C, elongation time = 1min, phusion.

We run the PCR product on 2 gels : -1% agarose for 1°&2° -2% agarose for 3°&4°


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