IGEM:Paris Bettencourt 2012/Notebooks/MAGE group/Auxotrophic

List of Auxotrophic Strains

==Alanine== There are multiple pathways for alanine biosynthesis, and no known complete auxotrophs exist. ==Arginine== Knockout in argA (Strains JW2786-1 and NK5992) ==Asparagine== Requires knockouts in both asnA and asnB (strain ER) ==Aspartic acid== Requires knockouts in both aspC and tyrB (strain DL39) ==Cysteine== Knockout in cysE (Strains JW3582-2 and JM15) ==Glutamic acid== Requires knockouts in both gltB and gdhA (strain PA340) ==Glutamine== Knockout in glnA (Strains JW3841-1 and M5004) ==Glycine== Knockout in glyA (Strains JW2535-1 and AT2457) ==Histidine== Knockout in hisB (Strains JW2004-1 and SB3930) ==Isoleucine== Knockout in ilvA (Strain JW3745-2 and AB1255) ==Leucine== Knockout in leuB (Strain JW5807-2 and CV514) ==Lysine== Knockout in lysA (Strain JW2806-1 and KL334) ==Methionine== Knockout in metA (Strain JW3973-1 and DL41) ==Phenylalanine== Knockout in pheA (Strain JW2580-1 and KA197) ==Proline== Knockout in proA (Strain JW0233-2 and NK5525) ==Serine== Knockout in serA (Strain JW2880-1 and JC158) ==Threonine== Knockout in thrC (Strain JW0003-2 and Gif 41) ==Tryptophan== Knockout in trpC (Strain JW1254-2 and CAG18455) ==Tyrosine== Knockout in tyrA (Strain JW2581-1 and N3087) ==Valine/Isoleucine/Leucine== Knockout in ilvD (Strain JW5605-1 and CAG18431)