Revision as of 09:37, 25 June 2012

List of Auxotrophic Strains

Alanine

There are multiple pathways for alanine biosynthesis, and no known complete auxotrophs exist.

Arginine

Knockout in argA (Strains JW2786-1 and NK5992)

Asparagine

Requires knockouts in both asnA and asnB (strain ER)

Aspartic acid

Requires knockouts in both aspC and tyrB (strain DL39)

Cysteine

Knockout in cysE (Strains JW3582-2 and JM15)

Glutamic acid

Requires knockouts in both gltB and gdhA (strain PA340)

Glutamine

Knockout in glnA (Strains JW3841-1 and M5004)

Glycine

Knockout in glyA (Strains JW2535-1 and AT2457)

Histidine

Knockout in hisB (Strains JW2004-1 and SB3930)

Isoleucine

Knockout in ilvA (Strain JW3745-2 and AB1255)

Leucine

Knockout in leuB (Strain JW5807-2 and CV514)

Lysine

Knockout in lysA (Strain JW2806-1 and KL334)

Methionine

Knockout in metA (Strain JW3973-1 and DL41)

Phenylalanine

Knockout in pheA (Strain JW2580-1 and KA197)

Proline

Knockout in proA (Strain JW0233-2 and NK5525)

Serine

Knockout in serA (Strain JW2880-1 and JC158)

Threonine

Knockout in thrC (Strain JW0003-2 and Gif 41)

Tryptophan

Knockout in trpC (Strain JW1254-2 and CAG18455)

Tyrosine

Knockout in tyrA (Strain JW2581-1 and N3087)

Valine/Isoleucine/Leucine

Knockout in ilvD (Strain JW5605-1 and CAG18431)

Organism: Escherichia coli K-12 substr. MG1655

Chromosome: Chromosome

Region: 3951501 - 3953351

  atgCCTAAGT ACCGTTCCGC CACCACCACT CATGGTCGTA ATATGGCGGG TGCTCGTGCG
  CTGTGGCGCG CCACCGGAAT GACCGACGCC GATTTCGGTA AGCCGATTAT CGCGGTTGTG
  AACTCGTTCA CCCAATTTGT ACCGGGTCAC GTCCATCTGC GCGATCTCGG TAAACTGGTC
  GCCGAACAAA TTGAAGCGGC TGGCGGCGTT GCCAAAGAGT TCAACACCAT TGCGGTGGAT
  GATGGGATTG CCATGGGCCA CGGGGGGATG CTTTATTCAC TGCCATCTCG CGAACTGATC
  GCTGATTCCG TTGAGTATAT GGTCAACGCC CACTGCGCCG ACGCCATGGT CTGCATCTCT
  AACTGCGACA AAATCACCCC GGGGATGCTG ATGGCTTCCC TGCGCCTGAA TATTCCGGTG
  ATCTTTGTTT CCGGCGGCCC GATGGAGGCC GGGAAAACCA AACTTTCCGA TCAGATCATC
  AAGCTCGATC TGGTTGATGC GATGATCCAG GGCGCAGACC CGAAAGTATC TGACTCCCAG
  AGCGATCAGG TTGAACGTTC CGCGTGTCCG ACCTGCGGTT CCTGCTCCGG GATGTTTACC
  GCTAACTCAA TGAACTGCCT GACCGAAGCG CTGGGCCTGT CGCAGCCGGG CAACGGCTCG
  CTGCTGGCAA CCCACGCCGA CCGTAAGCAG CTGTTCCTTA ATGCTGGTAA ACGCATTGTT
  GAATTGACCA AACGTTATTA CGAGCAAAAC GACGAAAGTG CACTGCCGCG TAATATCGCC
  AGTAAGGCGG CGTTTGAAAA CGCCATGACG CTGGATATCG CGATGGGTGG ATCGACTAAC
  ACCGTACTTC ACCTGCTGGC GGCGGCGCAG GAAGCGGAAA TCGACTTCAC CATGAGTGAT
  ATCGATAAGC TTTCCCGCAA GGTTCCACAG CTGTGTAAAG TTGCGCCGAG CACCCAGAAA
  TACCATATGG AAGATGTTCA CCGTGCTGGT GGTGTTATCG GTATTCTCGG CGAACTGGAT
  CGCGCGGGGT TACTGAACCG TGATGTGAAA AACGTACTTG GCCTGACGTT GCCGCAAACG
  CTGGAACAAT ACGACGTTAT GCTGACCCAG GATGACGCGG TAAAAAATAT GTTCCGCGCA
  GGTCCTGCAG GCATTCGTAC CACACAGGCA TTCTCGCAAG ATTGCCGTTG GGATACGCTG
  GACGACGATC GCGCCAATGG CTGTATCCGC TCGCTGGAAC ACGCCTACAG CAAAGACGGC
  GGCCTGGCGG TGCTCTACGG TAACTTTGCG GAAAACGGCT GCATCGTGAA AACGGCAGGC
  GTCGATGACA GCATCCTCAA ATTCACCGGC CCGGCGAAAG TGTACGAAAG CCAGGACGAT
  GCGGTAGAAG CGATTCTCGG CGGTAAAGTT GTCGCCGGAG ATGTGGTAGT AATTCGCTAT
  GAAGGCCCGA AAGGCGGTCC GGGGATGCAG GAAATGCTCT ACCCAACCAG CTTCCTGAAA
  TCAATGGGTC TCGGCAAAGC CTGTGCGCTG ATCACCGACG GTCGTTTCTC TGGTGGCACC
  TCTGGTCTTT CCATCGGCCA CGTCTCACCG GAAGCGGCAA GCGGCGGCAG CATTGGCCTG
  ATTGAAGATG GTGACCTGAT CGCTATCGAC ATCCCGAACC GTGGCATTCA GTTACAGGTA
  AGCGATGCCG AACTGGCGGC GCGTCGTGAA GCGCAGGACG CTCGAGGTGA CAAAGCCTGG
  ACGCCGAAAA ATCGTGAACG TCAGGTCTCC TTTGCCCTGC GTGCTTATGC CAGCCTGGCA
  ACCAGCGCCG ACAAAGGCGC GGTGCGCGAT AAATCGAAAC TGGGGGGTta a

IGEM:Paris Bettencourt 2012/Notebooks/MAGE group/Auxotrophic: Difference between revisions