IGEM:Paris Bettencourt 2012/Notebooks/MAGE group/Auxotrophic: Difference between revisions
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==Serine== | ==Serine== | ||
Knockout in '''serA''' (Strain JW2880-1 and JC158) | Knockout in '''serA''' (Strain JW2880-1 and JC158) | ||
Map Position: [3,055,200 <- 3,056,432] (65.85 centisomes) | |||
Length: 1233 bp / 410 aa | |||
==Threonine== | ==Threonine== | ||
Knockout in '''thrC''' (Strain JW0003-2 and Gif 41) | Knockout in '''thrC''' (Strain JW0003-2 and Gif 41) | ||
Revision as of 09:53, 25 June 2012
List of Auxotrophic Strains
Alanine
There are multiple pathways for alanine biosynthesis, and no known complete auxotrophs exist.
Arginine
Knockout in argA (Strains JW2786-1 and NK5992)
Asparagine
Requires knockouts in both asnA and asnB (strain ER)
Aspartic acid
Requires knockouts in both aspC and tyrB (strain DL39)
Cysteine
Knockout in cysE (Strains JW3582-2 and JM15)
Glutamic acid
Requires knockouts in both gltB and gdhA (strain PA340)
Glutamine
Knockout in glnA (Strains JW3841-1 and M5004)
Glycine
Knockout in glyA (Strains JW2535-1 and AT2457)
Histidine
Knockout in hisB (Strains JW2004-1 and SB3930)
Isoleucine
Knockout in ilvA (Strain JW3745-2 and AB1255)
Leucine
Knockout in leuB (Strain JW5807-2 and CV514)
Lysine
Knockout in lysA (Strain JW2806-1 and KL334)
Methionine
Knockout in metA (Strain JW3973-1 and DL41)
Phenylalanine
Knockout in pheA (Strain JW2580-1 and KA197)
Proline
Knockout in proA (Strain JW0233-2 and NK5525)
Serine
Knockout in serA (Strain JW2880-1 and JC158)
Map Position: [3,055,200 <- 3,056,432] (65.85 centisomes)
Length: 1233 bp / 410 aa
Threonine
Knockout in thrC (Strain JW0003-2 and Gif 41)
Map Position: [3,734 -> 5,020] (0.08 centisomes)
Length: 1287 bp / 428 aa
Tryptophan
Knockout in trpC (Strain JW1254-2 and CAG18455)
Map Position: [1,316,451 <- 1,317,809] (28.37 centisomes)
Length: 1359 bp / 452 aa
Tyrosine
- Knockout in tyrA (Strain JW2581-1 and N3087)
Organism: Escherichia coli K-12 substr. MG1655
Chromosome: Chromosome
Map Position: [2,736,970 <- 2,738,091] (58.99 centisomes)
Length: 1122 bp / 373 aa
atgGTTGCTG AATTGACCGC ATTACGCGAT CAAATTGATG AAGTCGATAA AGCGCTGCTG
AATTTATTAG CGAAGCGTCT GGAACTGGTT GCTGAAGTGG GCGAGGTGAA AAGCCGCTTT
GGACTGCCTA TTTATGTTCC GGAGCGCGAG GCATCTATGT TGGCCTCGCG TCGTGCAGAG
GCGGAAGCTC TGGGTGTACC GCCAGATCTG ATTGAGGATG TTTTGCGTCG GGTGATGCGT
GAATCTTACT CCAGTGAAAA CGACAAAGGA TTTAAAACAC TTTGTCCGTC ACTGCGTCCG
GTGGTTATCG TCGGCGGTGG CGGTCAGATG GGACGCCTGT TCGAGAAGAT GCTGACCCTC
TCGGGTTATC AGGTGCGGAT TCTGGAGCAA CATGACTGGG ATCGAGCGGC TGATATTGTT
GCCGATGCCG GAATGGTGAT TGTTAGTGTG CCAATCCACG TTACTGAGCA AGTTATTGGC
AAATTACCGC CTTTACCGAA AGATTGTATT CTGGTCGATC TGGCATCAGT GAAAAATGGG
CCATTACAGG CCATGCTGGT GGCGCATGAT GGTCCGGTGC TGGGGCTACA CCCGATGTTC
GGTCCGGACA GCGGTAGCCT GGCAAAGCAA GTTGTGGTCT GGTGTGATGG ACGTAAACCG
GAAGCATACC AATGGTTTCT GGAGCAAATT CAGGTCTGGG GCGCTCGGCT GCATCGTATT
AGCGCCGTCG AGCACGATCA GAATATGGCG TTTATTCAGG CACTGCGCCA CTTTGCTACT
TTTGCTTACG GGCTGCACCT GGCAGAAGAA AATGTTCAGC TTGAGCAACT TCTGGCGCTC
TCTTCGCCGA TTTACCGCCT TGAGCTGGCG ATGGTCGGGC GACTGTTTGC TCAGGATCCG
CAGCTTTATG CCGACATCAT TATGTCGTCA GAGCGTAATC TGGCGTTAAT CAAACGTTAC
TATAAGCGTT TCGGCGAGGC GATTGAGTTG CTGGAGCAGG GCGATAAGCA GGCGTTTATT
GACAGTTTCC GCAAGGTGGA GCACTGGTTC GGCGATTACG CACAGCGTTT TCAGAGTGAA
AGCCGCGTGT TATTGCGTCA GGCGAATGAC AATCGCCAGt aa
Valine/Isoleucine/Leucine
- Knockout in ilvD (Strain JW5605-1 and CAG18431)
Organism: Escherichia coli K-12 substr. MG1655
Chromosome: Chromosome
Map Position: [3,951,501 -> 3,953,351] (85.17 centisomes)
Length: 1851 bp / 616 aa
atgCCTAAGT ACCGTTCCGC CACCACCACT CATGGTCGTA ATATGGCGGG TGCTCGTGCG CTGTGGCGCG CCACCGGAAT GACCGACGCC GATTTCGGTA AGCCGATTAT CGCGGTTGTG AACTCGTTCA CCCAATTTGT ACCGGGTCAC GTCCATCTGC GCGATCTCGG TAAACTGGTC GCCGAACAAA TTGAAGCGGC TGGCGGCGTT GCCAAAGAGT TCAACACCAT TGCGGTGGAT GATGGGATTG CCATGGGCCA CGGGGGGATG CTTTATTCAC TGCCATCTCG CGAACTGATC GCTGATTCCG TTGAGTATAT GGTCAACGCC CACTGCGCCG ACGCCATGGT CTGCATCTCT AACTGCGACA AAATCACCCC GGGGATGCTG ATGGCTTCCC TGCGCCTGAA TATTCCGGTG ATCTTTGTTT CCGGCGGCCC GATGGAGGCC GGGAAAACCA AACTTTCCGA TCAGATCATC AAGCTCGATC TGGTTGATGC GATGATCCAG GGCGCAGACC CGAAAGTATC TGACTCCCAG AGCGATCAGG TTGAACGTTC CGCGTGTCCG ACCTGCGGTT CCTGCTCCGG GATGTTTACC GCTAACTCAA TGAACTGCCT GACCGAAGCG CTGGGCCTGT CGCAGCCGGG CAACGGCTCG CTGCTGGCAA CCCACGCCGA CCGTAAGCAG CTGTTCCTTA ATGCTGGTAA ACGCATTGTT GAATTGACCA AACGTTATTA CGAGCAAAAC GACGAAAGTG CACTGCCGCG TAATATCGCC AGTAAGGCGG CGTTTGAAAA CGCCATGACG CTGGATATCG CGATGGGTGG ATCGACTAAC ACCGTACTTC ACCTGCTGGC GGCGGCGCAG GAAGCGGAAA TCGACTTCAC CATGAGTGAT ATCGATAAGC TTTCCCGCAA GGTTCCACAG CTGTGTAAAG TTGCGCCGAG CACCCAGAAA TACCATATGG AAGATGTTCA CCGTGCTGGT GGTGTTATCG GTATTCTCGG CGAACTGGAT CGCGCGGGGT TACTGAACCG TGATGTGAAA AACGTACTTG GCCTGACGTT GCCGCAAACG CTGGAACAAT ACGACGTTAT GCTGACCCAG GATGACGCGG TAAAAAATAT GTTCCGCGCA GGTCCTGCAG GCATTCGTAC CACACAGGCA TTCTCGCAAG ATTGCCGTTG GGATACGCTG GACGACGATC GCGCCAATGG CTGTATCCGC TCGCTGGAAC ACGCCTACAG CAAAGACGGC GGCCTGGCGG TGCTCTACGG TAACTTTGCG GAAAACGGCT GCATCGTGAA AACGGCAGGC GTCGATGACA GCATCCTCAA ATTCACCGGC CCGGCGAAAG TGTACGAAAG CCAGGACGAT GCGGTAGAAG CGATTCTCGG CGGTAAAGTT GTCGCCGGAG ATGTGGTAGT AATTCGCTAT GAAGGCCCGA AAGGCGGTCC GGGGATGCAG GAAATGCTCT ACCCAACCAG CTTCCTGAAA TCAATGGGTC TCGGCAAAGC CTGTGCGCTG ATCACCGACG GTCGTTTCTC TGGTGGCACC TCTGGTCTTT CCATCGGCCA CGTCTCACCG GAAGCGGCAA GCGGCGGCAG CATTGGCCTG ATTGAAGATG GTGACCTGAT CGCTATCGAC ATCCCGAACC GTGGCATTCA GTTACAGGTA AGCGATGCCG AACTGGCGGC GCGTCGTGAA GCGCAGGACG CTCGAGGTGA CAAAGCCTGG ACGCCGAAAA ATCGTGAACG TCAGGTCTCC TTTGCCCTGC GTGCTTATGC CAGCCTGGCA ACCAGCGCCG ACAAAGGCGC GGTGCGCGAT AAATCGAAAC TGGGGGGTta a
