IGEM:Paris Bettencourt 2012/Notebooks/MAGE group/Auxotrophic

List of Auxotrophic Strains

Alanine

There are multiple pathways for alanine biosynthesis, and no known complete auxotrophs exist.

Arginine

Knockout in argA (Strains JW2786-1 and NK5992)

Asparagine

Requires knockouts in both asnA and asnB (strain ER)

Aspartic acid

Requires knockouts in both aspC and tyrB (strain DL39)

Cysteine

Knockout in cysE (Strains JW3582-2 and JM15)

Glutamic acid

Requires knockouts in both gltB and gdhA (strain PA340)

Glutamine

Knockout in glnA (Strains JW3841-1 and M5004)

Glycine

Knockout in glyA (Strains JW2535-1 and AT2457)

Histidine

Knockout in hisB (Strains JW2004-1 and SB3930)

Isoleucine

Knockout in ilvA (Strain JW3745-2 and AB1255)

Leucine

Knockout in leuB (Strain JW5807-2 and CV514)

Lysine

Knockout in lysA (Strain JW2806-1 and KL334)

Methionine

Knockout in metA (Strain JW3973-1 and DL41)

Phenylalanine

Knockout in pheA (Strain JW2580-1 and KA197)

Map Position: [2,735,767 -> 2,736,927] (58.96 centisomes)

Length: 1161 bp / 386 aa

Proline

Knockout in proA (Strain JW0233-2 and NK5525)

Map Position: [260,727 -> 261,980] (5.62 centisomes)

Length: 1254 bp / 417 a

Serine

Knockout in serA (Strain JW2880-1 and JC158)

Map Position: [3,055,200 <- 3,056,432] (65.85 centisomes)

Length: 1233 bp / 410 aa

Threonine

Knockout in thrC (Strain JW0003-2 and Gif 41)

Map Position: [3,734 -> 5,020] (0.08 centisomes)

Length: 1287 bp / 428 aa

Tryptophan

Knockout in trpC (Strain JW1254-2 and CAG18455)

Map Position: [1,316,451 <- 1,317,809] (28.37 centisomes)

Length: 1359 bp / 452 aa

Tyrosine

• Knockout in tyrA (Strain JW2581-1 and N3087)

Organism: Escherichia coli K-12 substr. MG1655

Chromosome: Chromosome

Map Position: [2,736,970 <- 2,738,091] (58.99 centisomes)

Length: 1122 bp / 373 aa

    atgGTTGCTG AATTGACCGC ATTACGCGAT CAAATTGATG AAGTCGATAA AGCGCTGCTG
    AATTTATTAG CGAAGCGTCT GGAACTGGTT GCTGAAGTGG GCGAGGTGAA AAGCCGCTTT
    GGACTGCCTA TTTATGTTCC GGAGCGCGAG GCATCTATGT TGGCCTCGCG TCGTGCAGAG
    GCGGAAGCTC TGGGTGTACC GCCAGATCTG ATTGAGGATG TTTTGCGTCG GGTGATGCGT
    GAATCTTACT CCAGTGAAAA CGACAAAGGA TTTAAAACAC TTTGTCCGTC ACTGCGTCCG
    GTGGTTATCG TCGGCGGTGG CGGTCAGATG GGACGCCTGT TCGAGAAGAT GCTGACCCTC
    TCGGGTTATC AGGTGCGGAT TCTGGAGCAA CATGACTGGG ATCGAGCGGC TGATATTGTT
    GCCGATGCCG GAATGGTGAT TGTTAGTGTG CCAATCCACG TTACTGAGCA AGTTATTGGC
    AAATTACCGC CTTTACCGAA AGATTGTATT CTGGTCGATC TGGCATCAGT GAAAAATGGG
    CCATTACAGG CCATGCTGGT GGCGCATGAT GGTCCGGTGC TGGGGCTACA CCCGATGTTC
    GGTCCGGACA GCGGTAGCCT GGCAAAGCAA GTTGTGGTCT GGTGTGATGG ACGTAAACCG
    GAAGCATACC AATGGTTTCT GGAGCAAATT CAGGTCTGGG GCGCTCGGCT GCATCGTATT
    AGCGCCGTCG AGCACGATCA GAATATGGCG TTTATTCAGG CACTGCGCCA CTTTGCTACT
    TTTGCTTACG GGCTGCACCT GGCAGAAGAA AATGTTCAGC TTGAGCAACT TCTGGCGCTC
    TCTTCGCCGA TTTACCGCCT TGAGCTGGCG ATGGTCGGGC GACTGTTTGC TCAGGATCCG
    CAGCTTTATG CCGACATCAT TATGTCGTCA GAGCGTAATC TGGCGTTAAT CAAACGTTAC
    TATAAGCGTT TCGGCGAGGC GATTGAGTTG CTGGAGCAGG GCGATAAGCA GGCGTTTATT
    GACAGTTTCC GCAAGGTGGA GCACTGGTTC GGCGATTACG CACAGCGTTT TCAGAGTGAA
    AGCCGCGTGT TATTGCGTCA GGCGAATGAC AATCGCCAGt aa

Valine/Isoleucine/Leucine

• Knockout in ilvD (Strain JW5605-1 and CAG18431)

Organism: Escherichia coli K-12 substr. MG1655

Chromosome: Chromosome

Map Position: [3,951,501 -> 3,953,351] (85.17 centisomes)

Length: 1851 bp / 616 aa

    TACGAGACAG ACACTGGGAG TAAATAAAGT atgCCTAAGT ACCGTTCCGC CACCACCACT
    CATGGTCGTA ATATGGCGGG TGCTCGTGCG CTGTGGCGCG CCACCGGAAT GACCGACGCC
    GATTTCGGTA AGCCGATTAT CGCGGTTGTG AACTCGTTCA CCCAATTTGT ACCGGGTCAC
    GTCCATCTGC GCGATCTCGG TAAACTGGTC GCCGAACAAA TTGAAGCGGC TGGCGGCGTT
    GCCAAAGAGT TCAACACCAT TGCGGTGGAT GATGGGATTG CCATGGGCCA CGGGGGGATG
    CTTTATTCAC TGCCATCTCG CGAACTGATC GCTGATTCCG TTGAGTATAT GGTCAACGCC
    CACTGCGCCG ACGCCATGGT CTGCATCTCT AACTGCGACA AAATCACCCC GGGGATGCTG
    ATGGCTTCCC TGCGCCTGAA TATTCCGGTG ATCTTTGTTT CCGGCGGCCC GATGGAGGCC
    GGGAAAACCA AACTTTCCGA TCAGATCATC AAGCTCGATC TGGTTGATGC GATGATCCAG
    GGCGCAGACC CGAAAGTATC TGACTCCCAG AGCGATCAGG TTGAACGTTC CGCGTGTCCG
    ACCTGCGGTT CCTGCTCCGG GATGTTTACC GCTAACTCAA TGAACTGCCT GACCGAAGCG
    CTGGGCCTGT CGCAGCCGGG CAACGGCTCG CTGCTGGCAA CCCACGCCGA CCGTAAGCAG
    CTGTTCCTTA ATGCTGGTAA ACGCATTGTT GAATTGACCA AACGTTATTA CGAGCAAAAC
    GACGAAAGTG CACTGCCGCG TAATATCGCC AGTAAGGCGG CGTTTGAAAA CGCCATGACG
    CTGGATATCG CGATGGGTGG ATCGACTAAC ACCGTACTTC ACCTGCTGGC GGCGGCGCAG
    GAAGCGGAAA TCGACTTCAC CATGAGTGAT ATCGATAAGC TTTCCCGCAA GGTTCCACAG
    CTGTGTAAAG TTGCGCCGAG CACCCAGAAA TACCATATGG AAGATGTTCA CCGTGCTGGT
    GGTGTTATCG GTATTCTCGG CGAACTGGAT CGCGCGGGGT TACTGAACCG TGATGTGAAA
    AACGTACTTG GCCTGACGTT GCCGCAAACG CTGGAACAAT ACGACGTTAT GCTGACCCAG
    GATGACGCGG TAAAAAATAT GTTCCGCGCA GGTCCTGCAG GCATTCGTAC CACACAGGCA
    TTCTCGCAAG ATTGCCGTTG GGATACGCTG GACGACGATC GCGCCAATGG CTGTATCCGC
    TCGCTGGAAC ACGCCTACAG CAAAGACGGC GGCCTGGCGG TGCTCTACGG TAACTTTGCG
    GAAAACGGCT GCATCGTGAA AACGGCAGGC GTCGATGACA GCATCCTCAA ATTCACCGGC
    CCGGCGAAAG TGTACGAAAG CCAGGACGAT GCGGTAGAAG CGATTCTCGG CGGTAAAGTT
    GTCGCCGGAG ATGTGGTAGT AATTCGCTAT GAAGGCCCGA AAGGCGGTCC GGGGATGCAG
    GAAATGCTCT ACCCAACCAG CTTCCTGAAA TCAATGGGTC TCGGCAAAGC CTGTGCGCTG
    ATCACCGACG GTCGTTTCTC TGGTGGCACC TCTGGTCTTT CCATCGGCCA CGTCTCACCG
    GAAGCGGCAA GCGGCGGCAG CATTGGCCTG ATTGAAGATG GTGACCTGAT CGCTATCGAC
    ATCCCGAACC GTGGCATTCA GTTACAGGTA AGCGATGCCG AACTGGCGGC GCGTCGTGAA
    GCGCAGGACG CTCGAGGTGA CAAAGCCTGG ACGCCGAAAA ATCGTGAACG TCAGGTCTCC
    TTTGCCCTGC GTGCTTATGC CAGCCTGGCA ACCAGCGCCG ACAAAGGCGC GGTGCGCGAT
    AAATCGAAAC TGGGGGGTta aTAATGGCTG ACTCGCAACC CCTGTCCGGT G