IGEM:Paris Bettencourt 2012/Notebooks/RE group: Difference between revisions
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*[[IGEM:Paris_Bettencourt_2012/Notebook/RE_group/Restriction_Enzymes_candidates | Restriction Enzymes candidates]] | *[[IGEM:Paris_Bettencourt_2012/Notebook/RE_group/Restriction_Enzymes_candidates | Restriction Enzymes candidates]] | ||
*[[Promotor Candidates]] | *[[IGEM:Paris_Bettencourt_2012/Notebook/RE_group/Promotor_Candidates | Promotor Candidates]] | ||
*[[Construction design]] | *[[IGEM:Paris_Bettencourt_2012/Notebook/RE_group/Construction_design | Construction design]] | ||
Revision as of 04:28, 27 June 2012
Inducible promoters candidates
Our main objective is to find inducible promoters that are not leaky.
P bad
Overview of PBAD Promoter Family: [1]
- Pbad Part:BBa_I13453
- Pbad+AraC Part:BBa_I0500
- Characterizationby the Cambridge 2009 team of different Pbad/araC promoters
- Plasmid: Part pSB3K3
P lac
- LacIq Part:BBa_K091111
- LacI Part: BBa_C0012
- Lac Operon Part:BBa_R0010
- Lac Operon Part(mutant of the BBa_R0010 part. Lower leakage, but also lower expression) BBa_K611024
P tet
- Characterizationby the EPF-Lausanne 2011 team
- Ptet Part:BBa_R0040
- TetR Part:BBa_C0040
Interesting Articles
- "In Vivo Induction Kinetics of the Arabinose Promoters in Escherichia coli" CASONYA M. JOHNSON AND ROBERT F. SCHLEIF* [2] (Pbad)
- "Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements."
Rolf Lutz and Hermann Bujard* [3] (Ptet)
- "Tightly regulated vectors for the cloning and expression of toxic genes". Larry C. Anthony*, Hideki Suzuki, Marcin Filutowicz [4] (Plac)
Links
- Codon optomozation: [5]
