IGEM:Paris Bettencourt 2012/Notebooks/RE group

• IGEM:Paris_Bettencourt_2012/Notebook/RE_group/Restriction_Enzymes_candidates Restriction Enzymes candidates
• Promotor Candidates
• Construction design

Inducible promoters candidates

Our main objective is to find inducible promoters that are not leaky.

P bad

Overview of PBAD Promoter Family: [1]

• Pbad Part:BBa_I13453
• Pbad+AraC Part:BBa_I0500
• Characterizationby the Cambridge 2009 team of different Pbad/araC promoters
• Plasmid: Part pSB3K3

P lac

• LacIq Part:BBa_K091111
• LacI Part: BBa_C0012
• Lac Operon Part:BBa_R0010
• Lac Operon Part(mutant of the BBa_R0010 part. Lower leakage, but also lower expression) BBa_K611024

P tet

• Characterizationby the EPF-Lausanne 2011 team
• Ptet Part:BBa_R0040
• TetR Part:BBa_C0040

Interesting Articles

• "In Vivo Induction Kinetics of the Arabinose Promoters in Escherichia coli" CASONYA M. JOHNSON AND ROBERT F. SCHLEIF* [2] (Pbad)
• "Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements."

Rolf Lutz and Hermann Bujard* [3] (Ptet)

• "Tightly regulated vectors for the cloning and expression of toxic genes". Larry C. Anthony*, Hideki Suzuki, Marcin Filutowicz [4] (Plac)

Links

1. Codon optomozation: [5]