IGEM:Paris Bettencourt 2012/Notebooks/RE group/Construction design: Difference between revisions
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# Codon optomozation: [https://bioinfo.invitrogen.com/geneartgenes/projectmgmt] | # Codon optomozation: [https://bioinfo.invitrogen.com/geneartgenes/projectmgmt] | ||
# ATG...TAA | # ATG...TAA | ||
# | # [https://salis.psu.edu/ Salis Lab Calculator] (not the strongest RBS) | ||
We are planing '''''FokI''''' restriction site to devide sequence (almost in the middle). | We are planing '''''FokI''''' restriction site to devide sequence (almost in the middle). | ||
Revision as of 04:59, 27 June 2012
Fse gene (gBlocks Gene Fragments)
- Gene sequence
- Check usage in E.Coli
- Check the lack of:
- EcoRI [E]
- Xbal [X]
- SpeI [S]
- PstI [P]
- FseI
- Codon optomozation: [1]
- ATG...TAA
- Salis Lab Calculator (not the strongest RBS)
We are planing FokI restriction site to devide sequence (almost in the middle).
