IGEM:Paris Bettencourt 2012/Notebooks/RE group/Construction design

Fse gene (gBlocks Gene Fragments)

1. Gene sequence
   • Full: [Frankia sp. Eul1b FseI restriction-modification system gene cluster, complete sequence]
   • Without methyltransferase: [Frankia sp. Eul1b FseI restriction-modification system gene cluster, complete sequence]
2. Check usage in E.Coli
3. Check the lack of:
   • EcoRI (E)
   • Xbal (X)
   • SpeI (S)
   • PstI (P)
   • FseI
4. Codon optomozation: [1]
5. ATG...TAA
6. Salis Lab Calculator (not the strongest RBS)

We are planing FokI restriction site to devide sequence (almost in the middle).