IGEM:Paris Bettencourt 2012/Notebooks/SID group

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Synthetic Import Domain

Colicin is a type of bacteriocin produced by and toxic to some strains of Escherichia coli. Colicins are released into the environment to reduce competition from other bacterial strains. Colicins bind to outer membrane receptors, using them to translocate to the cytoplasm or cytoplasmic membrane, where they exert their cytotoxic effect, including depolarisation of the cytoplasmic membrane, DNase activity, RNase activity, or inhibition of murein synthesis.

We are interested in two of them :

  • Colicin E2 (DNase activity, Tol-dependent)
  • Colicin D (RNase activity, TonB-dependent)

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