IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/annexe: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 16: Line 16:
=Sequences=
=Sequences=
===Prefix 1===
===Prefix 1===
Before sequences starting with ATG
<code>GAATTCGCGGCCGCTTCTAG</code>
<code>GAATTCGCGGCCGCTTCTAG</code>
===Prefix 2===
===Prefix 2===
Before sequences starting with '''no''' ATG
<code>GAATTCGCGGCCGCTTCTAGAG</code>
<code>GAATTCGCGGCCGCTTCTAGAG</code>
===Suffix===
===Suffix===

Revision as of 08:53, 2 July 2012

Notebook Design Roadmap Meetings and to-dos Protocols Bibliography Previous Biosafety iGEM projects


Semantic containment project

Annexes

Sequences

Prefix 1

Before sequences starting with ATG GAATTCGCGGCCGCTTCTAG

Prefix 2

Before sequences starting with no ATG GAATTCGCGGCCGCTTCTAGAG

Suffix

TACTAGTAGCGGCCGCTGCAG

Plasmid 1 : pSB3C5::S0/1/6

NB :

  • P- & -S means Prefix and Suffix added
  • S0 means : no serines changed
  • S1 means : 1 serine changed to TAG (amber codon)
  • S6 means : 6 serines changed to TAG


P-placI-RBS-lacZ_α_S0-T-S = S0


 GAATTCGCGGCCGCTTCTAGAGCGTTGACACCATCGAATGGCGCAAAACC
 TTTCGCGGTATGGCATGATAGCGCCCGGGAAAGAGGGGACAAATGACCAT
 GATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC
 CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC
 TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCG
 CAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGG
 TGCCGGAAAGCTGGCTGGAGTAATAAAAAAAAAAACCCCGCCCCTGACAG
 GGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAG

P-placI-RBS-lacZ_α_S1-T-S = S1

 GAATTCGCGGCCGCTTCTAGAGCGTTGACACCATCGAATGGCGCAAAACC
 TTTCGCGGTATGGCATGATAGCGCCCGGGAAAGAGGGGACAAATGACCAT
 GATTACGGATTAGCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC
 CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC
 TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCG
 CAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGG
 TGCCGGAAAGCTGGCTGGAGTAATAAAAAAAAAAACCCCGCCCCTGACAG
 GGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAG

P-placI-RBS-lacZ_α_S6-T-S = S6

 GAATTCGCGGCCGCTTCTAGAGCGTTGACACCATCGAATGGCGCAAAACC
 TTTCGCGGTATGGCATGATAGCGCCCGGGAAAGAGGGGACAAATGACCAT
 GATTACGGATTAGCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC
 CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCTAG
 TGGCGTAATTAGGAAGAGGCCCGCACCGATCGCCCTTAGCAACAGTTGCG
 CTAGCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGG
 TGCCGGAATAGTGGCTGGAGTAATAAAAAAAAAAACCCCGCCCCTGACAG
 GGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAG

Plasmid 2 : pSB1A3::supD_T1

BBa_K228001 - SupD-tRNA

 CAATTCGGAGAGATGCCGGAGCGGCTGAACGGACCGGTCTCTAAAACCGG
 AGTAGGGGCAACTCTACCGGGGGTTCAAATCCCCCTCTCTCCGCCACTAC
 AGATCCTTAGCGAAAGCTAAGGATTTTTTTTAAGCT

BBa_B1006 - Terminator 1

AAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTT

Scripts

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Paris_Bettencourt_2012/Notebooks/Semantic_group/annexe&oldid=611615"