IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/annexe: Difference between revisions
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*S6 means : 6 serines changed to TAG | *S6 means : 6 serines changed to TAG | ||
Geneious file : [[Image:LacZ_S0_1_6.geneious]] | Geneious file : [[Image:LacZ_S0_1_6.geneious]] (right click, save under...) | ||
===P-placI-RBS-lacZ_α_S0-T-S = S0=== | ===P-placI-RBS-lacZ_α_S0-T-S = S0=== |
Revision as of 09:07, 5 July 2012
Notebook | Design | Roadmap | Meetings and to-dos | Protocols | Bibliography | Previous Biosafety iGEM projects |
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Annexes
Sequences
Prefix 1
Before sequences starting with ATG
GAATTCGCGGCCGCTTCTAG
Prefix 2
Before sequences starting with no ATG
GAATTCGCGGCCGCTTCTAGAG
Suffix
TACTAGTAGCGGCCGCTGCAG
Plasmid 1 : pSB3C5::S0/1/6
- placI : BBa_K091110
- RBS : BBa_J61100
- lacZ-alphapart : BBa_I732006
- Terminator : BBa_B1006
NB :
- P- & -S means Prefix and Suffix added
- S0 means : no serines changed
- S1 means : 1 serine changed to TAG (amber codon)
- S6 means : 6 serines changed to TAG
Geneious file : File:LacZ S0 1 6.geneious (right click, save under...)
P-placI-RBS-lacZ_α_S0-T-S = S0
GAATTCGCGGCCGCTTCTAGAGCGTTGACACCATCGAATGGCGCAAAACC
TTTCGCGGTATGGCATGATAGCGCCCGGGAAAGAGGGGACAAATGACCAT
GATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC
CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC
TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCG
CAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGG
TGCCGGAAAGCTGGCTGGAGTAATAAAAAAAAAAACCCCGCCCCTGACAG
GGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAG
P-placI-RBS-lacZ_α_S1-T-S = S1
GAATTCGCGGCCGCTTCTAGAGCGTTGACACCATCGAATGGCGCAAAACC
TTTCGCGGTATGGCATGATAGCGCCCGGGAAAGAGGGGACAAATGACCAT
GATTACGGATTAGCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC
CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC
TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCG
CAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGG
TGCCGGAAAGCTGGCTGGAGTAATAAAAAAAAAAACCCCGCCCCTGACAG
GGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAG
P-placI-RBS-lacZ_α_S6-T-S = S6
GAATTCGCGGCCGCTTCTAGAGCGTTGACACCATCGAATGGCGCAAAACC
TTTCGCGGTATGGCATGATAGCGCCCGGGAAAGAGGGGACAAATGACCAT
GATTACGGATTAGCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC
CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCTAG
TGGCGTAATTAGGAAGAGGCCCGCACCGATCGCCCTTAGCAACAGTTGCG
CTAGCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGG
TGCCGGAATAGTGGCTGGAGTAATAAAAAAAAAAACCCCGCCCCTGACAG
GGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAG
Plasmid 2 : pSB1A3::supD_T1
BBa_K228001 - SupD-tRNA
CAATTCGGAGAGATGCCGGAGCGGCTGAACGGACCGGTCTCTAAAACCGG
AGTAGGGGCAACTCTACCGGGGGTTCAAATCCCCCTCTCTCCGCCACTAC
AGATCCTTAGCGAAAGCTAAGGATTTTTTTTAAGCT
BBa_B1006 - Terminator 1
AAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTT