IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/annexe: Difference between revisions

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*S6 means : 6 serines changed to TAG
*S6 means : 6 serines changed to TAG


Geneious file : [[Image:LacZ_S0_1_6.geneious]]
Geneious file : [[Image:LacZ_S0_1_6.geneious]] (right click, save under...)


===P-placI-RBS-lacZ_α_S0-T-S = S0===
===P-placI-RBS-lacZ_α_S0-T-S = S0===

Revision as of 09:07, 5 July 2012

Notebook Design Roadmap Meetings and to-dos Protocols Bibliography Previous Biosafety iGEM projects


Semantic containment project

Annexes


Sequences

Prefix 1

Before sequences starting with ATG GAATTCGCGGCCGCTTCTAG

Prefix 2

Before sequences starting with no ATG GAATTCGCGGCCGCTTCTAGAG

Suffix

TACTAGTAGCGGCCGCTGCAG

Plasmid 1 : pSB3C5::S0/1/6

NB :

  • P- & -S means Prefix and Suffix added
  • S0 means : no serines changed
  • S1 means : 1 serine changed to TAG (amber codon)
  • S6 means : 6 serines changed to TAG

Geneious file : File:LacZ S0 1 6.geneious (right click, save under...)

P-placI-RBS-lacZ_α_S0-T-S = S0


 GAATTCGCGGCCGCTTCTAGAGCGTTGACACCATCGAATGGCGCAAAACC
 TTTCGCGGTATGGCATGATAGCGCCCGGGAAAGAGGGGACAAATGACCAT
 GATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC
 CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC
 TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCG
 CAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGG
 TGCCGGAAAGCTGGCTGGAGTAATAAAAAAAAAAACCCCGCCCCTGACAG
 GGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAG

P-placI-RBS-lacZ_α_S1-T-S = S1

 GAATTCGCGGCCGCTTCTAGAGCGTTGACACCATCGAATGGCGCAAAACC
 TTTCGCGGTATGGCATGATAGCGCCCGGGAAAGAGGGGACAAATGACCAT
 GATTACGGATTAGCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC
 CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC
 TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCG
 CAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGG
 TGCCGGAAAGCTGGCTGGAGTAATAAAAAAAAAAACCCCGCCCCTGACAG
 GGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAG

P-placI-RBS-lacZ_α_S6-T-S = S6

 GAATTCGCGGCCGCTTCTAGAGCGTTGACACCATCGAATGGCGCAAAACC
 TTTCGCGGTATGGCATGATAGCGCCCGGGAAAGAGGGGACAAATGACCAT
 GATTACGGATTAGCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC
 CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCTAG
 TGGCGTAATTAGGAAGAGGCCCGCACCGATCGCCCTTAGCAACAGTTGCG
 CTAGCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGG
 TGCCGGAATAGTGGCTGGAGTAATAAAAAAAAAAACCCCGCCCCTGACAG
 GGCGGGGTTTTTTTTTACTAGTAGCGGCCGCTGCAG

Plasmid 2 : pSB1A3::supD_T1

BBa_K228001 - SupD-tRNA

 CAATTCGGAGAGATGCCGGAGCGGCTGAACGGACCGGTCTCTAAAACCGG
 AGTAGGGGCAACTCTACCGGGGGTTCAAATCCCCCTCTCTCCGCCACTAC
 AGATCCTTAGCGAAAGCTAAGGATTTTTTTTAAGCT

BBa_B1006 - Terminator 1

AAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTT

Scripts