IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/annexe: Difference between revisions
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| Line 35: | Line 35: | ||
Geneious file : [[Image:Semantic.geneious]] (right click, save under...) | Geneious file : [[Image:Semantic.geneious]] (right click, save under...) | ||
===KanR all serines changed=== | ===P-KanR all serines changed-S=== | ||
<code> | <code> | ||
GAATTCGCGGCCGCTTCTAGATGTAGCATATTCAACGGGAAACGTAGTGC | GAATTCGCGGCCGCTTCTAGATGTAGCATATTCAACGGGAAACGTAGTGC | ||
| Line 55: | Line 55: | ||
TTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATACTAGTAGCGGCC | TTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATACTAGTAGCGGCC | ||
GCTGCAG | GCTGCAG | ||
</code> | </code> | ||
=== Fragment 1 === | === Fragment 1 === | ||
<code> | <code> | ||
Latest revision as of 02:32, 9 July 2012
| Notebook | Design | Roadmap | Meetings and to-dos | Protocols | Bibliography | Previous Biosafety iGEM projects |
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Annexes
Sequences
Prefix 1
Before sequences starting with ATG
GAATTCGCGGCCGCTTCTAG
Prefix 2
Before sequences starting with no ATG
GAATTCGCGGCCGCTTCTAGAG
Suffix
TACTAGTAGCGGCCGCTGCAG
Plasmid 1 : pSB3C5::S0/1/6
- KanR : [1]
NB :
- P- & -S means Prefix and Suffix added
Geneious file : File:Semantic.geneious (right click, save under...)
P-KanR all serines changed-S
GAATTCGCGGCCGCTTCTAGATGTAGCATATTCAACGGGAAACGTAGTGC
TAGAGGCCGCGATTAAATTAGAACATGGATGCTGATTTATATGGGTATAA
ATGGGCTCGCGATAATGTCGGGCAATAGGGTGCGACAATCTATCGATTGT
ATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTTAG
GTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGA
ATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATG
CATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTA
GAAGAATATCCTGATTAGGGTGAAAATATTGTTGATGCGCTGGCAGTGTT
CCTGCGCCGGTTGCATTAGATTCCTGTTTGTAATTGTCCTTTTAACTAGG
ATCGCGTATTTCGTCTCGCTCAGGCGCAATAGCGAATGAATAACGGTTTG
GTTGATGCGTAGGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACA
AGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTAGCCGGATTAGGTCG
TCACTCATGGTGATTTCTAGCTTGATAACCTTATTTTTGACGAGGGGAAA
TTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCA
GGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTAGCCTTAGTTAC
AGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAA
TTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATACTAGTAGCGGCC
GCTGCAG
Fragment 1
GAATTCGCGGCCGCTTCTAGATGTAGCATATTCAACGGGAAACGTAGTGC
TAGAGGCCGCGATTAAATTAGAACATGGATGCTGATTTATATGGGTATAA
ATGGGCTCGCGATAATGTCGGGCAATAGGGTGCGACAATCTATCGATTGT
ATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTTAG
GTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGA
ATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATG
CATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTA
GAAGAATATCCTGATTAGGGTGAAAATATTGTTGATGCGCTGGCAGTGTT
CCTGCGCCGGTTGCATTAGATTCCTGTTTGTAATTGTCCTTTTAACTAGG
ATCGCGTATTTCGTCTCGCTCAGGCGCAATAGCGAATGAATAACG
Fragment 2
GATTAGGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTT
GCATTAGATTCCTGTTTGTAATTGTCCTTTTAACTAGGATCGCGTATTTC
GTCTCGCTCAGGCGCAATAGCGAATGAATAACGGTTTGGTTGATGCGTAG
GATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGA
AATGCATAAGCTTTTGCCATTCTAGCCGGATTAGGTCGTCACTCATGGTG
ATTTCTAGCTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGT
ATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCAT
CCTATGGAACTGCCTCGGTGAGTTTTAGCCTTAGTTACAGAAACGGCTTT
TTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCAT
TTGATGCTCGATGAGTTTTTCTAATACTAGTAGCGGCCGCTGCAG
Plasmid 2 : pSB1A3::supD_T1
BBa_K228001 - SupD-tRNA
CAATTCGGAGAGATGCCGGAGCGGCTGAACGGACCGGTCTCTAAAACCGG
AGTAGGGGCAACTCTACCGGGGGTTCAAATCCCCCTCTCTCCGCCACTAC
AGATCCTTAGCGAAAGCTAAGGATTTTTTTTAAGCT
BBa_B1006 - Terminator 1
AAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTTTTT
